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Assay Detail

CHEMBL4714158

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-nisoxetine from human NET expressed in HEK293 cell membranes incubated for 60 mins by scintillation counting method
39
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent noradrenaline transporter (CHEMBL222)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Bicyclic Diazepinones as Dual Ligands of the α2δ-1 Subunit of Voltage-Gated Calcium Channels and the Norepinephrine Transporter.
Díaz JL,Cuevas F,Pazos G,Álvarez-Bercedo P,Oliva AI,Sarmentero MÁ,Font D,Jiménez-Aquino A,Morón M,Port A,Pascual R,Dordal A,Portillo-Salido E,Reinoso RF,Vela JM,Almansa C
J Med Chem (2021) 64 : 2167 -2185
PubMed 33591743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 39 6.87 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4750411 1 8.40
CHEMBL4779959 1 7.54
CHEMBL383921 1 7.41
CHEMBL4790212 1 7.39
CHEMBL4745144 1 7.23
CHEMBL4764742 1 7.22
CHEMBL4743954 1 7.16
CHEMBL4748644 1 7.12
CHEMBL4740331 1 7.11
CHEMBL4744681 1 7.09
CHEMBL4761288 1 6.98
CHEMBL4785616 1 6.74
CHEMBL4744472 1 6.52
CHEMBL4747442 1 6.47
CHEMBL4746870 1 6.45
CHEMBL4753306 1 6.42
CHEMBL4792765 1 6.42
CHEMBL4757946 1 6.25
CHEMBL4794892 1 6.21
CHEMBL4756591 1 6.08
CHEMBL4748656 1 6.00
CHEMBL4790988 1 -
CHEMBL4744000 1 -
CHEMBL4746713 1 -
CHEMBL4782498 1 -
CHEMBL4790433 1 -
CHEMBL4747320 1 -
CHEMBL4761964 1 -
CHEMBL4755653 1 -
CHEMBL4744196 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4750411 Ki = 4.0 nM 8.40
CHEMBL4779959 Ki = 29.0 nM 7.54
CHEMBL383921 Ki = 39.0 nM 7.41
CHEMBL4790212 Ki = 41.0 nM 7.39
CHEMBL4745144 Ki = 59.0 nM 7.23
CHEMBL4764742 Ki = 60.0 nM 7.22
CHEMBL4743954 Ki = 69.0 nM 7.16
CHEMBL4748644 Ki = 75.0 nM 7.12
CHEMBL4740331 Ki = 77.0 nM 7.11
CHEMBL4744681 Ki = 82.0 nM 7.09
CHEMBL4761288 Ki = 105.0 nM 6.98
CHEMBL4785616 Ki = 182.0 nM 6.74
CHEMBL4744472 Ki = 305.0 nM 6.52
CHEMBL4747442 Ki = 341.0 nM 6.47
CHEMBL4746870 Ki = 356.0 nM 6.45
CHEMBL4753306 Ki = 382.0 nM 6.42
CHEMBL4792765 Ki = 383.0 nM 6.42
CHEMBL4757946 Ki = 556.0 nM 6.25
CHEMBL4794892 Ki = 610.0 nM 6.21
CHEMBL4756591 Ki = 842.0 nM 6.08
CHEMBL4748656 Ki = 995.0 nM 6.00
CHEMBL4744196 Ki > 10000.0 nM -
CHEMBL1059 PREGABALIN Ki > 10000.0 nM -
CHEMBL4799404 Ki > 1000.0 nM -
CHEMBL4750920 Ki > 10000.0 nM -
CHEMBL4762006 Ki > 10000.0 nM -
CHEMBL4799032 Ki > 10000.0 nM -
CHEMBL4748556 Ki > 10000.0 nM -
CHEMBL4798113 Ki > 10000.0 nM -
CHEMBL4787604 Ki > 10000.0 nM -
CHEMBL4791954 Ki > 10000.0 nM -
CHEMBL4755653 Ki > 10000.0 nM -
CHEMBL4761964 Ki > 10000.0 nM -
CHEMBL4747320 Ki > 1000.0 nM -
CHEMBL4790433 Ki > 10000.0 nM -
CHEMBL4782498 Ki > 1000.0 nM -
CHEMBL4746713 Ki > 1000.0 nM -
CHEMBL4744000 Ki > 10000.0 nM -
CHEMBL4790988 Ki > 10000.0 nM -