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Assay Detail

CHEMBL4715063

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at recombinant rat TRPA1 stably expressed in HEK293 cells assessed as increase in intracellular calcium release by Fluo-4AM dye based fluorescence assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Structural and in Vitro Functional Characterization of a Menthyl TRPM8 Antagonist Indicates Species-Dependent Regulation.
Journigan VB,Alarcón-Alarcón D,Feng Z,Wang Y,Liang T,Dawley DC,Amin ARMR,Montano C,Van Horn WD,Xie XQ,Ferrer-Montiel A,Fernández-Carvajal A
ACS Med Chem Lett (2021) 12 : 758 -767
PubMed 34055223 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 6.38 6.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2387742 CANNABIDIVARIN 2.0 1 6.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2387742 CANNABIDIVARIN EC50 = 420.0 nM 6.38