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Assay Detail

CHEMBL4715191

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity in CXCR2 (unknown origin) expressed in human HEK293 cells co-expressing Galpha16 assessed as reduction in calcium immobilization pretreated with Fluo-4AM for 45 mins followed by compound addition and measured after 10 mins by Fluo-4AM dye based fluorescence assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-X-C chemokine receptor type 2 (CHEMBL2434)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 1,5-Dihydro-4H-imidazol-4-one Derivatives as Potent, Selective Antagonists of CXC Chemokine Receptor 2.
Che J,Wang Z,Shen Z,Zhuang W,Ying H,Hu Y,Hu Y,Xie X,Dong X
ACS Med Chem Lett (2021) 12 : 836 -845
PubMed 34055234 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.50 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4786074 1 8.22
CHEMBL4763312 1 8.05
CHEMBL4742368 1 8.00
CHEMBL216981 NAVARIXIN ANHYDROUS 2.0 1 8.00
CHEMBL4757674 1 7.82
CHEMBL4746698 1 7.54
CHEMBL4747035 1 7.37
CHEMBL4752486 1 7.27
CHEMBL4793352 1 7.17
CHEMBL4741864 1 7.01
CHEMBL4750465 1 6.85
CHEMBL4800297 1 6.57
CHEMBL4746803 1 6.52
CHEMBL4799848 1 6.52
CHEMBL4786309 1 6.33
CHEMBL4757462 1 6.04
CHEMBL4797191 1 5.66
CHEMBL4755486 1 5.55
CHEMBL4762597 1 5.51
CHEMBL4745933 1 5.40
CHEMBL4536494 1 4.82
CHEMBL4777637 1 4.63
CHEMBL4760919 1 4.60
CHEMBL4765070 1 4.53
CHEMBL4797342 1 -
CHEMBL4781979 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4786074 IC50 = 6.0 nM 8.22
CHEMBL4763312 IC50 = 9.0 nM 8.05
CHEMBL4742368 IC50 = 10.0 nM 8.00
CHEMBL216981 NAVARIXIN ANHYDROUS IC50 = 10.0 nM 8.00
CHEMBL4757674 IC50 = 15.0 nM 7.82
CHEMBL4746698 IC50 = 29.0 nM 7.54
CHEMBL4747035 IC50 = 43.0 nM 7.37
CHEMBL4752486 IC50 = 54.0 nM 7.27
CHEMBL4793352 IC50 = 68.0 nM 7.17
CHEMBL4741864 IC50 = 98.0 nM 7.01
CHEMBL4750465 IC50 = 140.0 nM 6.85
CHEMBL4800297 IC50 = 269.0 nM 6.57
CHEMBL4746803 IC50 = 305.0 nM 6.52
CHEMBL4799848 IC50 = 305.0 nM 6.52
CHEMBL4786309 IC50 = 470.0 nM 6.33
CHEMBL4757462 IC50 = 915.0 nM 6.04
CHEMBL4797191 IC50 = 2190.0 nM 5.66
CHEMBL4755486 IC50 = 2800.0 nM 5.55
CHEMBL4762597 IC50 = 3100.0 nM 5.51
CHEMBL4745933 IC50 = 4000.0 nM 5.40
CHEMBL4536494 IC50 = 15000.0 nM 4.82
CHEMBL4777637 IC50 = 23600.0 nM 4.63
CHEMBL4760919 IC50 = 25000.0 nM 4.60
CHEMBL4765070 IC50 = 29700.0 nM 4.53
CHEMBL4797342 IC50 > 100000.0 nM -
CHEMBL4781979 IC50 > 100000.0 nM -