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Assay Detail

CHEMBL4715396

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at CCR2 in human THP1 cells assessed as reduction in MCP1-induced chemotaxis measured after 30 mins by calcein-AM dye based fluorescence assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type THP-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-C chemokine receptor type 2 (CHEMBL4015)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of BMS-753426: A Potent Orally Bioavailable Antagonist of CC Chemokine Receptor 2.
Yang MG,Xiao Z,Zhao R,Tebben AJ,Wang B,Cherney RJ,Batt DG,Brown GD,Cvijic ME,Duncia JV,Gallela MA,Gardner DS,Khandelwal P,Malley MF,Pang J,Rose AV,Santella JB,Sarjeant AA,Xu S,Mathur A,Mandlekar S,Vuppugalla R,Zhao Q,Carter PH
ACS Med Chem Lett (2021) 12 : 969 -975
PubMed 34141082 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 8.79 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4764460 1 9.52
CHEMBL4790208 1 9.22
CHEMBL4442783 1 9.15
CHEMBL4781426 1 9.10
CHEMBL4757857 1 8.80
CHEMBL4760098 1 8.77
CHEMBL4757452 1 8.52
CHEMBL4741305 1 8.35
CHEMBL4742683 1 7.70
CHEMBL4746510 1 -
CHEMBL4760566 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4764460 IC50 = 0.3 nM 9.52
CHEMBL4790208 IC50 = 0.6 nM 9.22
CHEMBL4442783 IC50 = 0.7 nM 9.15
CHEMBL4781426 IC50 = 0.8 nM 9.10
CHEMBL4757857 IC50 = 1.6 nM 8.80
CHEMBL4760098 IC50 = 1.7 nM 8.77
CHEMBL4757452 IC50 = 3.0 nM 8.52
CHEMBL4741305 IC50 = 4.5 nM 8.35
CHEMBL4742683 IC50 = 20.0 nM 7.70
CHEMBL4746510 IC50 - -
CHEMBL4760566 IC50 - -