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Assay Detail

CHEMBL4718381

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human erythrocytes mu-calpain using Pep1 as substrate incubated for 30 mins under shaking condition in presence of CaCl2 by fluorescence assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Erythrocyte
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Calpain-1 catalytic subunit (CHEMBL3891)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Neuroprotective effect of synthetic chalcone derivatives as competitive dual inhibitors against μ-calpain and cathepsin B through the downregulation of tau phosphorylation and insoluble Aβ peptide formation.
Jeon KH,Lee E,Jun KY,Eom JE,Kwak SY,Na Y,Kwon Y
Eur J Med Chem (2016) 121 : 433 -444
PubMed 27318120 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.04 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL423112 1 7.00
CHEMBL92708 CALPEPTIN 1 6.53
CHEMBL2368566 1 6.40
CHEMBL4765091 1 4.99
CHEMBL4799634 1 4.89
CHEMBL4786246 1 4.86
CHEMBL4756611 1 4.77
CHEMBL4764053 1 4.72
CHEMBL4740912 1 4.72
CHEMBL4746320 1 4.62
CHEMBL4740843 1 4.39
CHEMBL4780290 1 4.33
CHEMBL63440 ALOXISTATIN 2.0 1 4.21
CHEMBL4743183 1 4.16
CHEMBL490920 1 -
CHEMBL4789024 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL423112 IC50 = 99.0 nM 7.00
CHEMBL92708 CALPEPTIN IC50 = 297.0 nM 6.53
CHEMBL2368566 IC50 = 397.1 nM 6.40
CHEMBL4765091 IC50 = 10230.0 nM 4.99
CHEMBL4799634 IC50 = 12980.0 nM 4.89
CHEMBL4786246 IC50 = 13700.0 nM 4.86
CHEMBL4756611 IC50 = 16810.0 nM 4.77
CHEMBL4764053 IC50 = 19060.0 nM 4.72
CHEMBL4740912 IC50 = 18830.0 nM 4.72
CHEMBL4746320 IC50 = 23820.0 nM 4.62
CHEMBL4740843 IC50 = 40870.0 nM 4.39
CHEMBL4780290 IC50 = 46570.0 nM 4.33
CHEMBL63440 ALOXISTATIN IC50 = 62260.0 nM 4.21
CHEMBL4743183 IC50 = 69590.0 nM 4.16
CHEMBL490920 IC50 > 100000.0 nM -
CHEMBL4789024 IC50 > 50000.0 nM -