Assay Detail
Binding
CHEMBL4718937
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at Go2-q-coupled rat EBI2 expressed in COS7 cell membranes assessed as stimulation of [35S]GTPgammaS binding preincubated for 20 mins followed by [35S]GTPgammaS addition and measured after 1 hr by scintillation counting analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Cell Type | COS7 |
| Subcellular | Membrane |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
G-protein coupled receptor 183 (CHEMBL4802001) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Fluoro analogs of bioactive oxy-sterols: Synthesis of an EBI2 agonist with enhanced metabolic stability.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 3 | 8.56 | 8.97 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4779254 | — | — | 1 | 8.97 |
| CHEMBL4788348 | — | — | 1 | 8.88 |
| CHEMBL4757291 | — | — | 1 | 7.84 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4779254 | — | EC50 | = | 1.08 | nM | 8.97 |
| CHEMBL4788348 | — | EC50 | = | 1.31 | nM | 8.88 |
| CHEMBL4757291 | — | EC50 | = | 14.4 | nM | 7.84 |