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Assay Detail

CHEMBL4719215

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PDE-4 in human PBMC assessed as LPS-induced TNFalpha production incubated for 15 mins followed by LPS stimulation and measured after 18 hrs by ELISA relative to control
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type PBMC
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

In vivo effective dibenzo[b,d]furan-1-yl-thiazoles as novel PDE-4 inhibitors.
Balasubramanian G,Narayanan S,Andiappan L,Sappanimuthu T,Thirunavukkarasu S,Sundaram S,Natarajan S,Sivaraman N,Rajagopal S,Nazumudeen FAA,Saxena S,Vishwakarma SL,Narayanan S,Sharma GVR,Srinivasan CV,Kilambi N
Bioorg Med Chem (2016) 24 : 5702 -5716
PubMed 27713015 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 6.98 8.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 8.37
CHEMBL4744412 1 7.74
CHEMBL4756900 1 7.56
CHEMBL4791975 1 7.51
CHEMBL4799096 1 7.42
CHEMBL4764245 1 7.40
CHEMBL4747406 1 7.26
CHEMBL514800 APREMILAST 4.0 1 7.11
CHEMBL4753261 1 6.41
CHEMBL4744894 1 5.76
CHEMBL4787884 1 5.66
CHEMBL4750720 1 5.56
CHEMBL4756735 1 -
CHEMBL4758625 1 -
CHEMBL4752629 1 -
CHEMBL4798712 1 -
CHEMBL4753966 1 -
CHEMBL4781742 1 -
CHEMBL4754301 1 -
CHEMBL4744425 1 -
CHEMBL4796802 1 -
CHEMBL4761698 1 -
CHEMBL4799146 1 -
CHEMBL4751333 1 -
CHEMBL4762734 1 -
CHEMBL4755546 1 -
CHEMBL4790625 1 -
CHEMBL4754022 1 -
CHEMBL4748998 1 -
CHEMBL4760887 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 4.3 nM 8.37
CHEMBL4744412 IC50 = 18.4 nM 7.74
CHEMBL4756900 IC50 = 27.7 nM 7.56
CHEMBL4791975 IC50 = 31.0 nM 7.51
CHEMBL4799096 IC50 = 37.7 nM 7.42
CHEMBL4764245 IC50 = 39.6 nM 7.40
CHEMBL4747406 IC50 = 54.4 nM 7.26
CHEMBL514800 APREMILAST IC50 = 77.0 nM 7.11
CHEMBL4753261 IC50 = 385.7 nM 6.41
CHEMBL4744894 IC50 = 1753.0 nM 5.76
CHEMBL4787884 IC50 = 2208.0 nM 5.66
CHEMBL4750720 IC50 = 2780.0 nM 5.56
CHEMBL4781742 IC50 - -
CHEMBL4753966 IC50 - -
CHEMBL4798712 IC50 - -
CHEMBL4752629 IC50 - -
CHEMBL4758625 IC50 - -
CHEMBL4756735 IC50 - -
CHEMBL4750994 IC50 - -
CHEMBL4762087 IC50 - -
CHEMBL4782414 IC50 - -
CHEMBL4789336 IC50 - -
CHEMBL4760774 IC50 - -
CHEMBL4784700 IC50 - -
CHEMBL4754301 IC50 - -
CHEMBL4744425 IC50 - -
CHEMBL4796802 IC50 - -
CHEMBL4761698 IC50 - -
CHEMBL4799146 IC50 - -
CHEMBL4751333 IC50 - -
CHEMBL4762734 IC50 - -
CHEMBL4755546 IC50 - -
CHEMBL4790625 IC50 - -
CHEMBL4754022 IC50 - -
CHEMBL4748998 IC50 - -
CHEMBL4760887 IC50 - -