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Assay Detail

CHEMBL4719947

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-LY459477 from recombinant human mGlu2 receptor expressed in hamster AV12 cell membranes co-expressing rat EAAT1 incubated for 90 mins by top count scintillation method
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type AV12
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 2 (CHEMBL5137)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (1S,2R,3S,4S,5R,6R)-2-Amino-3-[(3,4-difluorophenyl)sulfanylmethyl]-4-hydroxy-bicyclo[3.1.0]hexane-2,6-dicarboxylic Acid Hydrochloride (LY3020371·HCl): A Potent, Metabotropic Glutamate 2/3 Receptor Antagonist with Antidepressant-Like Activity.
Chappell MD,Li R,Smith SC,Dressman BA,Tromiczak EG,Tripp AE,Blanco MJ,Vetman T,Quimby SJ,Matt J,Britton TC,Fivush AM,Schkeryantz JM,Mayhugh D,Erickson JA,Bures MG,Jaramillo C,Carpintero M,Diego JE,Barberis M,Garcia-Cerrada S,Soriano JF,Antonysamy S,Atwell S,MacEwan I,Condon B,Sougias C,Wang J,Zhang A,Conners K,Groshong C,Wasserman SR,Koss JW,Witkin JM,Li X,Overshiner C,Wafford KA,Seidel W,Wang XS,Heinz BA,Swanson S,Catlow JT,Bedwell DW,Monn JA,Mitch CH,Ornstein PL
J Med Chem (2016) 59 : 10974 -10993
PubMed 28002967 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.53 8.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL432038 1 8.37
CHEMBL4759992 1 8.28
CHEMBL4751065 1 8.28
CHEMBL4748699 1 8.08
CHEMBL4780402 1 8.05
CHEMBL4746125 1 8.04
CHEMBL4776989 1 7.98
CHEMBL4757159 1 7.85
CHEMBL4760856 1 7.69
CHEMBL4759209 1 7.67
CHEMBL4756556 1 7.62
CHEMBL4781638 1 7.51
CHEMBL4784701 1 7.36
CHEMBL4778355 1 6.57
CHEMBL4779554 1 6.57
CHEMBL4755204 1 6.14
CHEMBL4749728 1 5.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL432038 Ki = 4.3 nM 8.37
CHEMBL4759992 Ki = 5.2 nM 8.28
CHEMBL4751065 Ki = 5.3 nM 8.28
CHEMBL4748699 Ki = 8.4 nM 8.08
CHEMBL4780402 Ki = 8.9 nM 8.05
CHEMBL4746125 Ki = 9.2 nM 8.04
CHEMBL4776989 Ki = 10.4 nM 7.98
CHEMBL4757159 Ki = 14.2 nM 7.85
CHEMBL4760856 Ki = 20.5 nM 7.69
CHEMBL4759209 Ki = 21.4 nM 7.67
CHEMBL4756556 Ki = 24.0 nM 7.62
CHEMBL4781638 Ki = 30.9 nM 7.51
CHEMBL4784701 Ki = 43.7 nM 7.36
CHEMBL4778355 Ki = 268.0 nM 6.57
CHEMBL4779554 Ki = 271.0 nM 6.57
CHEMBL4755204 Ki = 728.0 nM 6.14
CHEMBL4749728 Ki = 1200.0 nM 5.92