Assay Detail
Binding
CHEMBL4722622
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of C-terminal His-tagged recombinant human MIF tautomerase activity expressed in Escherichia coli BL21 using 4-hydroxyphenyl pyruvic acid as substrate preincubated for 15 mins followed by substrate addition and measured for 10 mins in presence of 0.5 mM EDTA by UV absorbance based Cheng-Prusoff equation analysis
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-activity relationships for binding of 4-substituted triazole-phenols to macrophage migration inhibitory factor (MIF).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 18 | 4.93 | 6.36 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3953102 | — | — | 1 | 6.36 |
| CHEMBL4741927 | — | — | 1 | 6.02 |
| CHEMBL4745151 | — | — | 1 | 5.48 |
| CHEMBL4751935 | — | — | 1 | 5.30 |
| CHEMBL4749241 | — | — | 1 | 5.19 |
| CHEMBL4754122 | — | — | 1 | 5.16 |
| CHEMBL4763772 | — | — | 1 | 5.16 |
| CHEMBL4755471 | — | — | 1 | 4.77 |
| CHEMBL4800056 | — | — | 1 | 4.72 |
| CHEMBL4782511 | — | — | 1 | 4.64 |
| CHEMBL4752116 | — | — | 1 | 4.64 |
| CHEMBL4756250 | — | — | 1 | 4.58 |
| CHEMBL4756731 | — | — | 1 | 4.55 |
| CHEMBL4742375 | — | — | 1 | 4.52 |
| CHEMBL4795431 | — | — | 1 | 4.50 |
| CHEMBL4743992 | — | — | 1 | 4.44 |
| CHEMBL4745143 | — | — | 1 | 4.37 |
| CHEMBL210858 | — | — | 1 | 4.36 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3953102 | — | Ki | = | 440.0 | nM | 6.36 |
| CHEMBL4741927 | — | Ki | = | 960.0 | nM | 6.02 |
| CHEMBL4745151 | — | Ki | = | 3300.0 | nM | 5.48 |
| CHEMBL4751935 | — | Ki | = | 5000.0 | nM | 5.30 |
| CHEMBL4749241 | — | Ki | = | 6500.0 | nM | 5.19 |
| CHEMBL4754122 | — | Ki | = | 6900.0 | nM | 5.16 |
| CHEMBL4763772 | — | Ki | = | 6900.0 | nM | 5.16 |
| CHEMBL4755471 | — | Ki | = | 17000.0 | nM | 4.77 |
| CHEMBL4800056 | — | Ki | = | 19000.0 | nM | 4.72 |
| CHEMBL4782511 | — | Ki | = | 23000.0 | nM | 4.64 |
| CHEMBL4752116 | — | Ki | = | 23000.0 | nM | 4.64 |
| CHEMBL4756250 | — | Ki | = | 26000.0 | nM | 4.58 |
| CHEMBL4756731 | — | Ki | = | 28000.0 | nM | 4.55 |
| CHEMBL4742375 | — | Ki | = | 30000.0 | nM | 4.52 |
| CHEMBL4795431 | — | Ki | = | 32000.0 | nM | 4.50 |
| CHEMBL4743992 | — | Ki | = | 36000.0 | nM | 4.44 |
| CHEMBL4745143 | — | Ki | = | 43000.0 | nM | 4.37 |
| CHEMBL210858 | — | Ki | = | 44000.0 | nM | 4.36 |