Assay Detail
Binding
CHEMBL4722633
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human MIF tautomerase activity expressed in Escherichia coli using 4-hydroxyphenyl pyruvic acid as substrate preincubated for 30 mins followed by substrate addition and measured for 175 secs
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure-activity relationships for binding of 4-substituted triazole-phenols to macrophage migration inhibitory factor (MIF).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 6.18 | 7.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3917143 | — | — | 1 | 7.24 |
| CHEMBL3953102 | — | — | 1 | 6.23 |
| CHEMBL4751935 | — | — | 1 | 5.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3917143 | — | Ki | = | 57.0 | nM | 7.24 |
| CHEMBL3953102 | — | Ki | = | 590.0 | nM | 6.23 |
| CHEMBL4751935 | — | Ki | = | 8800.0 | nM | 5.06 |