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Assay Detail

CHEMBL4723161

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV 3C-like protease expressed in Escherichia coli BL21 (DE3) using ABCYL-KTSAVLQSGFRKME-EDANS as substrate preincubated for 1 hr followed by substrate addition and measured after 16 hrs by FRET assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome-related coronavirus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Chinese Therapeutic Strategy for Fighting COVID-19 and Potential Small-Molecule Inhibitors against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
Choudhry N,Zhao X,Xu D,Zanin M,Chen W,Yang Z,Chen J
J Med Chem (2020) 63 : 13205 -13227
PubMed 32845145 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.49 4.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL395990 RHOIFOLIN 1 4.56
CHEMBL611029 HERBACETIN 1 4.48
CHEMBL445978 PECTOLINARIN 1 4.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL395990 RHOIFOLIN IC50 = 27400.0 nM 4.56
CHEMBL611029 HERBACETIN IC50 = 33100.0 nM 4.48
CHEMBL445978 PECTOLINARIN IC50 = 37700.0 nM 4.42