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Assay Detail

CHEMBL4723565

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mTORC1 in human A2058 cells assessed as reduction in RPS6 phosphorylation at S235/236 residue incubated for 1 hrs by Western blot analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A2058
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

4-(Difluoromethyl)-5-(4-((3R,5S)-3,5-dimethylmorpholino)-6-((R)-3-methylmorpholino)-1,3,5-triazin-2-yl)pyridin-2-amine (PQR626), a Potent, Orally Available, and Brain-Penetrant mTOR Inhibitor for the Treatment of Neurological Disorders.
Borsari C,Keles E,Rageot D,Treyer A,Bohnacker T,Bissegger L,De Pascale M,Melone A,Sriramaratnam R,Beaufils F,Hamburger M,Hebeisen P,Löscher W,Fabbro D,Hillmann P,Wymann MP
J Med Chem (2020) 63 : 13595 -13617
PubMed 33166139 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.66 7.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4741511 1 7.21
CHEMBL4749352 1 7.15
CHEMBL4777159 1 6.87
CHEMBL4758329 1 6.86
CHEMBL4287799 1 6.86
CHEMBL4792469 1 6.72
CHEMBL4757223 1 6.62
CHEMBL4749250 1 6.62
CHEMBL4290128 1 6.58
CHEMBL4779306 1 6.41
CHEMBL4752162 1 6.39
CHEMBL4764200 1 6.37
CHEMBL4746047 1 6.33
CHEMBL4753323 1 6.25
CHEMBL4783277 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4741511 IC50 = 61.0 nM 7.21
CHEMBL4749352 IC50 = 71.0 nM 7.15
CHEMBL4777159 IC50 = 136.0 nM 6.87
CHEMBL4758329 IC50 = 137.0 nM 6.86
CHEMBL4287799 IC50 = 138.0 nM 6.86
CHEMBL4792469 IC50 = 192.0 nM 6.72
CHEMBL4757223 IC50 = 241.0 nM 6.62
CHEMBL4749250 IC50 = 238.0 nM 6.62
CHEMBL4290128 IC50 = 261.0 nM 6.58
CHEMBL4779306 IC50 = 392.0 nM 6.41
CHEMBL4752162 IC50 = 407.0 nM 6.39
CHEMBL4764200 IC50 = 426.0 nM 6.37
CHEMBL4746047 IC50 = 471.0 nM 6.33
CHEMBL4753323 IC50 = 561.0 nM 6.25
CHEMBL4783277 IC50 > 1000.0 nM -