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Assay Detail

CHEMBL4723567

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of alexa fluor 647-labeled kinase tracer 314 binding to recombinant human N-terminal GST-tagged mTOR (1360 to 2549 residues) expressed in baculovirus expression system measured after 60 mins by TR-FRET based LanthaScreen Eu kinase binding assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-(Difluoromethyl)-5-(4-((3R,5S)-3,5-dimethylmorpholino)-6-((R)-3-methylmorpholino)-1,3,5-triazin-2-yl)pyridin-2-amine (PQR626), a Potent, Orally Available, and Brain-Penetrant mTOR Inhibitor for the Treatment of Neurological Disorders.
Borsari C,Keles E,Rageot D,Treyer A,Bohnacker T,Bissegger L,De Pascale M,Melone A,Sriramaratnam R,Beaufils F,Hamburger M,Hebeisen P,Löscher W,Fabbro D,Hillmann P,Wymann MP
J Med Chem (2020) 63 : 13595 -13617
PubMed 33166139 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.76 8.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4749352 1 8.44
CHEMBL4741511 1 8.33
CHEMBL4777159 1 8.19
CHEMBL4287799 1 8.10
CHEMBL4792469 1 7.96
CHEMBL4758329 1 7.96
CHEMBL4290128 1 7.85
CHEMBL4749250 1 7.75
CHEMBL4764200 1 7.64
CHEMBL4779306 1 7.60
CHEMBL4746047 1 7.55
CHEMBL4753323 1 7.38
CHEMBL4783277 1 7.30
CHEMBL4757223 1 7.17
CHEMBL4752162 1 7.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4749352 Ki = 3.6 nM 8.44
CHEMBL4741511 Ki = 4.7 nM 8.33
CHEMBL4777159 Ki = 6.4 nM 8.19
CHEMBL4287799 Ki = 8.0 nM 8.10
CHEMBL4792469 Ki = 11.0 nM 7.96
CHEMBL4758329 Ki = 11.0 nM 7.96
CHEMBL4290128 Ki = 14.0 nM 7.85
CHEMBL4749250 Ki = 18.0 nM 7.75
CHEMBL4764200 Ki = 23.0 nM 7.64
CHEMBL4779306 Ki = 25.0 nM 7.60
CHEMBL4746047 Ki = 28.0 nM 7.55
CHEMBL4753323 Ki = 42.0 nM 7.38
CHEMBL4783277 Ki = 50.0 nM 7.30
CHEMBL4757223 Ki = 67.0 nM 7.17
CHEMBL4752162 Ki = 73.0 nM 7.14