Assay Detail
Binding
CHEMBL4729575
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human HDAC8 expressed in HEK293/T17 cells pre-incubated for 10 mins before Boc-Lys(TFA)-AM substrate addition and measured after 30 mins by fluorescence-based assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Rational Design of Suprastat: A Novel Selective Histone Deacetylase 6 Inhibitor with the Ability to Potentiate Immunotherapy in Melanoma Models.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 6.21 | 6.32 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4177129 | — | — | 1 | 6.32 |
| CHEMBL4777296 | — | — | 1 | 6.30 |
| CHEMBL4759584 | — | — | 1 | 6.21 |
| CHEMBL2179618 | NEXTURASTAT A | — | 1 | 6.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4177129 | — | IC50 | = | 478.0 | nM | 6.32 |
| CHEMBL4777296 | — | IC50 | = | 498.0 | nM | 6.30 |
| CHEMBL4759584 | — | IC50 | = | 614.0 | nM | 6.21 |
| CHEMBL2179618 | NEXTURASTAT A | IC50 | = | 988.0 | nM | 6.00 |