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Assay Detail

CHEMBL4730563

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFRvIII mutant in human U87 cells assessed as phosphorylation after 1 hr by immunoblotting analysis
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U87
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Development of a Potent Brain-Penetrant EGFR Tyrosine Kinase Inhibitor against Malignant Brain Tumors.
Tsang JE,Urner LM,Kim G,Chow K,Baufeld L,Faull K,Cloughesy TF,Clark PM,Jung ME,Nathanson DA
ACS Med Chem Lett (2020) 11 : 1799 -1809
PubMed 33062157 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.32 8.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4743220 1 8.35
CHEMBL4744655 1 8.21
CHEMBL4791756 1 7.97
CHEMBL553 ERLOTINIB 4.0 1 7.90
CHEMBL4783428 1 7.79
CHEMBL4740063 1 7.66
CHEMBL4786961 1 7.56
CHEMBL4762453 1 7.51
CHEMBL4750375 1 7.49
CHEMBL4794223 1 7.44
CHEMBL4744207 1 7.40
CHEMBL1198370 1 7.37
CHEMBL4759031 1 7.35
CHEMBL4743464 1 7.26
CHEMBL4793453 1 7.24
CHEMBL4759650 1 7.12
CHEMBL4778159 1 7.08
CHEMBL4453068 1 6.72
CHEMBL4786797 1 5.64
CHEMBL4793944 1 5.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4743220 IC50 = 4.48 nM 8.35
CHEMBL4744655 IC50 = 6.21 nM 8.21
CHEMBL4791756 IC50 = 10.8 nM 7.97
CHEMBL553 ERLOTINIB IC50 = 12.5 nM 7.90
CHEMBL4783428 IC50 = 16.2 nM 7.79
CHEMBL4740063 IC50 = 22.0 nM 7.66
CHEMBL4786961 IC50 = 27.6 nM 7.56
CHEMBL4762453 IC50 = 30.8 nM 7.51
CHEMBL4750375 IC50 = 32.2 nM 7.49
CHEMBL4794223 IC50 = 36.1 nM 7.44
CHEMBL4744207 IC50 = 40.1 nM 7.40
CHEMBL1198370 IC50 = 43.1 nM 7.37
CHEMBL4759031 IC50 = 44.8 nM 7.35
CHEMBL4743464 IC50 = 54.8 nM 7.26
CHEMBL4793453 IC50 = 57.8 nM 7.24
CHEMBL4759650 IC50 = 75.4 nM 7.12
CHEMBL4778159 IC50 = 83.5 nM 7.08
CHEMBL4453068 IC50 = 188.4 nM 6.72
CHEMBL4786797 IC50 = 2312.0 nM 5.64
CHEMBL4793944 IC50 = 3846.0 nM 5.42