Assay Detail
Functional
CHEMBL4730979
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Antiviral activity against Hepatitis B virus infected in human HepaRG cells assessed as inhibition of HBV DNA replication under CYP450 restriction pre-treated for 1 hr with CYP450 inhibitors ABT and SKF-525A on day 5 post-infection followed by removal of media and supplemented with fresh media containing diluted compound and further incubated for 3 days then followed by treatment for 1 hr with CYP450 inhibitors ABT and SKF-525A on day 8 post-infection followed by removal of media and supplemented with fresh media containing diluted compound and further incubated for 3 days in presence of 25 uM SKF-525A and measured on day 11 post-infection by chemiluminescence immunostaining assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Hepatitis B virus |
| Cell Type | HepaRG |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
P450s under Restriction (PURE) Screen Using HepaRG and Primary Human Hepatocytes for Discovery of Novel HBV Antivirals.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 6.84 | 7.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3742114 | — | — | 1 | 7.96 |
| CHEMBL4796398 | — | — | 1 | 6.70 |
| CHEMBL605300 | — | — | 1 | 6.58 |
| CHEMBL316306 | — | — | 1 | 6.11 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3742114 | — | EC50 | = | 11.0 | nM | 7.96 |
| CHEMBL4796398 | — | EC50 | = | 200.0 | nM | 6.70 |
| CHEMBL605300 | — | EC50 | = | 260.0 | nM | 6.58 |
| CHEMBL316306 | — | EC50 | = | 780.0 | nM | 6.11 |