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Assay Detail

CHEMBL4734035

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at adrenergic alpha 2 receptor in human brain membrane assessed as inhibition of UK14304-induced stimulation of [35S]GTPgammaS binding by liquid scintillation spectroscopy (Rvb = 1.77 +/- 0.16 uM)
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Tissue Brain
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Adrenergic receptor alpha-2 (CHEMBL2095158)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Di-aryl guanidinium derivatives: Towards improved α2-Adrenergic affinity and antagonist activity.
McMullan M,Kelly B,Mihigo HB,Keogh AP,Rodriguez F,Brocos-Mosquera I,García-Bea A,Miranda-Azpiazu P,Callado LF,Rozas I
Eur J Med Chem (2021) 209 : 112947 -112947
PubMed 33139112 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 4.64 5.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4787567 1 5.38
CHEMBL4795780 1 4.99
CHEMBL4763113 1 4.94
CHEMBL3216452 1 4.70
CHEMBL4755913 1 4.52
CHEMBL4745647 1 4.51
CHEMBL4799288 1 4.40
CHEMBL4798553 1 4.28
CHEMBL10332 1 4.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4787567 EC50 = 4140.0 nM 5.38
CHEMBL4795780 EC50 = 10200.0 nM 4.99
CHEMBL4763113 EC50 = 11600.0 nM 4.94
CHEMBL3216452 EC50 = 20200.0 nM 4.70
CHEMBL4755913 EC50 = 30000.0 nM 4.52
CHEMBL4745647 EC50 = 30800.0 nM 4.51
CHEMBL4799288 EC50 = 39900.0 nM 4.40
CHEMBL4798553 EC50 = 52300.0 nM 4.28
CHEMBL10332 EC50 = 87200.0 nM 4.06