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Assay Detail

CHEMBL4766270

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-TAK-875 from human recombinant full-length GPR40 expressed in human HEK293 cell membrane incubated for 2 hrs by solid scintillation counting method
34
Total Activities
32
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Free fatty acid receptor 1 (CHEMBL4422)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery, Preclinical, and Early Clinical Development of Potent and Selective GPR40 Agonists for the Treatment of Type 2 Diabetes Mellitus (LY2881835, LY2922083, and LY2922470).
Hamdouchi C,Kahl SD,Patel Lewis A,Cardona GR,Zink RW,Chen K,Eessalu TE,Ficorilli JV,Marcelo MC,Otto KA,Wilbur KL,Lineswala JP,Piper JL,Coffey DS,Sweetana SA,Haas JV,Brooks DA,Pratt EJ,Belin RM,Deeg MA,Ma X,Cannady EA,Johnson JT,Yumibe NP,Chen Q,Maiti P,Montrose-Rafizadeh C,Chen Y,Reifel Miller A
J Med Chem (2016) 59 : 10891 -10916
PubMed 27749056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 7.90 8.96
Activity 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4787010 1 8.96
CHEMBL4793371 1 8.89
CHEMBL4797858 1 8.82
CHEMBL4798993 1 8.82
CHEMBL1829173 1 8.66
CHEMBL4781604 1 8.60
CHEMBL4788206 1 8.57
CHEMBL4785057 1 8.46
CHEMBL4797124 1 8.46
CHEMBL4798023 1 8.40
CHEMBL4800563 1 8.39
CHEMBL4791842 1 8.34
CHEMBL3915620 LY2881835 1.0 2 8.33
CHEMBL4782004 1 8.32
CHEMBL1829174 FASIGLIFAM 3.0 1 8.11
CHEMBL4787498 2 8.09
CHEMBL4780641 1 7.96
CHEMBL4787628 1 7.89
CHEMBL4786335 1 7.85
CHEMBL4799453 1 7.80
CHEMBL4792075 1 7.70
CHEMBL4784555 1 7.54
CHEMBL4783033 1 7.23
CHEMBL4783190 1 7.17
CHEMBL4778264 1 7.10
CHEMBL4794233 1 7.00
CHEMBL4790921 1 6.92
CHEMBL4795763 1 6.89
CHEMBL4794904 1 6.70
CHEMBL4792027 1 6.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4787010 Ki = 1.1 nM 8.96
CHEMBL4793371 Ki = 1.3 nM 8.89
CHEMBL4797858 Ki = 1.5 nM 8.82
CHEMBL4798993 Ki = 1.5 nM 8.82
CHEMBL1829173 Ki = 2.2 nM 8.66
CHEMBL4781604 Ki = 2.5 nM 8.60
CHEMBL4788206 Ki = 2.7 nM 8.57
CHEMBL4785057 Ki = 3.5 nM 8.46
CHEMBL4797124 Ki = 3.5 nM 8.46
CHEMBL4798023 Ki = 4.0 nM 8.40
CHEMBL4800563 Ki = 4.1 nM 8.39
CHEMBL4791842 Ki = 4.6 nM 8.34
CHEMBL3915620 LY2881835 Ki = 4.7 nM 8.33
CHEMBL4782004 Ki = 4.8 nM 8.32
CHEMBL1829174 FASIGLIFAM Ki = 7.7 nM 8.11
CHEMBL4787498 Ki = 8.2 nM 8.09
CHEMBL4780641 Ki = 11.0 nM 7.96
CHEMBL4787628 Ki = 13.0 nM 7.89
CHEMBL4786335 Ki = 14.0 nM 7.85
CHEMBL4799453 Ki = 16.0 nM 7.80
CHEMBL4792075 Ki = 20.0 nM 7.70
CHEMBL4784555 Ki = 29.0 nM 7.54
CHEMBL4783033 Ki = 59.0 nM 7.23
CHEMBL4783190 Ki = 68.0 nM 7.17
CHEMBL4778264 Ki = 79.0 nM 7.10
CHEMBL4794233 Ki = 100.0 nM 7.00
CHEMBL4790921 Ki = 120.0 nM 6.92
CHEMBL4795763 Ki = 130.0 nM 6.89
CHEMBL4794904 Ki = 200.0 nM 6.70
CHEMBL4792027 Ki = 310.0 nM 6.51
CHEMBL4786299 Ki = 410.0 nM 6.39
CHEMBL3915620 LY2881835 Activity - -
CHEMBL4787498 Activity - -
CHEMBL3955132 Activity - -