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Assay Detail

CHEMBL4766273

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human recombinant full-length Prolink-tagged GPR40 fused with EA-tagged beta-arrestin expressed in human HEK293 cells assessed as induction of beta-arrestin recruitment measured after 90 mins by beta-galactosidase based PathHunter assay
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Free fatty acid receptor 1 (CHEMBL4422)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery, Preclinical, and Early Clinical Development of Potent and Selective GPR40 Agonists for the Treatment of Type 2 Diabetes Mellitus (LY2881835, LY2922083, and LY2922470).
Hamdouchi C,Kahl SD,Patel Lewis A,Cardona GR,Zink RW,Chen K,Eessalu TE,Ficorilli JV,Marcelo MC,Otto KA,Wilbur KL,Lineswala JP,Piper JL,Coffey DS,Sweetana SA,Haas JV,Brooks DA,Pratt EJ,Belin RM,Deeg MA,Ma X,Cannady EA,Johnson JT,Yumibe NP,Chen Q,Maiti P,Montrose-Rafizadeh C,Chen Y,Reifel Miller A
J Med Chem (2016) 59 : 10891 -10916
PubMed 27749056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 32 7.62 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4787010 1 8.96
CHEMBL4797858 1 8.82
CHEMBL4793371 1 8.77
CHEMBL4788206 1 8.54
CHEMBL4785057 1 8.48
CHEMBL4798993 1 8.48
CHEMBL4797124 1 8.41
CHEMBL4782004 1 8.28
CHEMBL3955132 1 8.15
CHEMBL4787498 1 8.10
CHEMBL4800563 1 8.09
CHEMBL3915620 LY2881835 1.0 1 8.06
CHEMBL4798023 1 8.03
CHEMBL4781604 1 8.00
CHEMBL4791842 1 7.96
CHEMBL1829173 1 7.96
CHEMBL1829174 FASIGLIFAM 3.0 1 7.92
CHEMBL4786335 1 7.60
CHEMBL4799453 1 7.58
CHEMBL4787628 1 7.44
CHEMBL4780641 1 7.25
CHEMBL4783033 1 7.24
CHEMBL4792075 1 7.21
CHEMBL4795763 1 7.08
CHEMBL4784555 1 7.00
CHEMBL4790921 1 6.77
CHEMBL4783190 1 6.47
CHEMBL4794233 1 6.28
CHEMBL4792027 1 6.27
CHEMBL4778264 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4787010 EC50 = 1.1 nM 8.96
CHEMBL4797858 EC50 = 1.5 nM 8.82
CHEMBL4793371 EC50 = 1.7 nM 8.77
CHEMBL4788206 EC50 = 2.9 nM 8.54
CHEMBL4785057 EC50 = 3.3 nM 8.48
CHEMBL4798993 EC50 = 3.3 nM 8.48
CHEMBL4797124 EC50 = 3.9 nM 8.41
CHEMBL4782004 EC50 = 5.3 nM 8.28
CHEMBL3955132 EC50 = 7.0 nM 8.15
CHEMBL4787498 EC50 = 7.9 nM 8.10
CHEMBL4800563 EC50 = 8.2 nM 8.09
CHEMBL3915620 LY2881835 EC50 = 8.7 nM 8.06
CHEMBL4798023 EC50 = 9.3 nM 8.03
CHEMBL4781604 EC50 = 10.0 nM 8.00
CHEMBL1829173 EC50 = 11.0 nM 7.96
CHEMBL4791842 EC50 = 11.0 nM 7.96
CHEMBL1829174 FASIGLIFAM EC50 = 12.0 nM 7.92
CHEMBL4786335 EC50 = 25.0 nM 7.60
CHEMBL4799453 EC50 = 26.0 nM 7.58
CHEMBL4787628 EC50 = 36.0 nM 7.44
CHEMBL4780641 EC50 = 56.0 nM 7.25
CHEMBL4783033 EC50 = 58.0 nM 7.24
CHEMBL4792075 EC50 = 62.0 nM 7.21
CHEMBL4795763 EC50 = 84.0 nM 7.08
CHEMBL4784555 EC50 = 100.0 nM 7.00
CHEMBL4790921 EC50 = 170.0 nM 6.77
CHEMBL4783190 EC50 = 340.0 nM 6.47
CHEMBL4794233 EC50 = 520.0 nM 6.28
CHEMBL4792027 EC50 = 540.0 nM 6.27
CHEMBL4778264 EC50 = 600.0 nM 6.22
CHEMBL4786299 EC50 = 680.0 nM 6.17
CHEMBL4794904 EC50 = 740.0 nM 6.13