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Assay Detail

CHEMBL4766281

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-(S)-2-(6-((2',6'-dimethyl-4'-(3-(methylsulfonyl)propoxy)biphenyl-3-yl)methoxy)-2,3-dihydrobenzofuran-3-yl)acetic acid from human recombinant full-length GPR40 expressed in human HEK293 cell membrane assessed as dissociation constant measured after 2 hrs by radioligand competitive assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Subcellular Cell membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Free fatty acid receptor 1 (CHEMBL4422)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery, Preclinical, and Early Clinical Development of Potent and Selective GPR40 Agonists for the Treatment of Type 2 Diabetes Mellitus (LY2881835, LY2922083, and LY2922470).
Hamdouchi C,Kahl SD,Patel Lewis A,Cardona GR,Zink RW,Chen K,Eessalu TE,Ficorilli JV,Marcelo MC,Otto KA,Wilbur KL,Lineswala JP,Piper JL,Coffey DS,Sweetana SA,Haas JV,Brooks DA,Pratt EJ,Belin RM,Deeg MA,Ma X,Cannady EA,Johnson JT,Yumibe NP,Chen Q,Maiti P,Montrose-Rafizadeh C,Chen Y,Reifel Miller A
J Med Chem (2016) 59 : 10891 -10916
PubMed 27749056 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 9 8.24 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1829173 1 8.72
CHEMBL4782004 1 8.70
CHEMBL4791842 1 8.66
CHEMBL3915620 LY2881835 1.0 1 8.60
CHEMBL4787498 1 8.46
CHEMBL1829174 FASIGLIFAM 3.0 1 8.35
CHEMBL4780641 1 8.02
CHEMBL4778264 1 7.43
CHEMBL4783190 1 7.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1829173 Kd = 1.9 nM 8.72
CHEMBL4782004 Kd = 2.0 nM 8.70
CHEMBL4791842 Kd = 2.2 nM 8.66
CHEMBL3915620 LY2881835 Kd = 2.5 nM 8.60
CHEMBL4787498 Kd = 3.5 nM 8.46
CHEMBL1829174 FASIGLIFAM Kd = 4.5 nM 8.35
CHEMBL4780641 Kd = 9.5 nM 8.02
CHEMBL4778264 Kd = 37.0 nM 7.43
CHEMBL4783190 Kd = 58.0 nM 7.24