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Assay Detail

CHEMBL4766673

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human RET using KKKSPGEYVNIEFG as substrate incubated for 15 mins followed by Mg/ATP addition and measured after 40 mins by [gamma-33P]-ATP assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proto-oncogene tyrosine-protein kinase receptor Ret (CHEMBL2041)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural optimization and structure-activity relationship studies of N-phenyl-7,8-dihydro-6H-pyrimido[5,4-b][1,4]oxazin-4-amine derivatives as a new class of inhibitors of RET and its drug resistance mutants.
Yang J,Chen K,Zhang G,Yang QY,Li YS,Huang SZ,Wang YL,Yang W,Jiang XJ,Yan HX,Zhu JQ,Xiang R,Luo YF,Li WM,Wei YQ,Li LL,Yang SY
Eur J Med Chem (2018) 143 : 1148 -1164
PubMed 29133048 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 6.74 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1336 SORAFENIB 4.0 1 8.05
CHEMBL24828 VANDETANIB 4.0 1 8.05
CHEMBL4779887 1 8.00
CHEMBL4793380 1 8.00
CHEMBL4789515 1 8.00
CHEMBL2105717 CABOZANTINIB 4.0 1 7.89
CHEMBL4784865 1 7.68
CHEMBL4784000 1 7.66
CHEMBL4797687 1 7.60
CHEMBL4798046 1 7.30
CHEMBL4794949 1 7.11
CHEMBL4797171 1 6.75
CHEMBL4793991 1 6.64
CHEMBL4793949 1 6.51
CHEMBL4786825 1 6.49
CHEMBL4798842 1 6.49
CHEMBL4789912 1 6.11
CHEMBL4786580 1 6.06
CHEMBL4798574 1 6.03
CHEMBL4792943 1 5.97
CHEMBL4797671 1 5.83
CHEMBL4794893 1 5.65
CHEMBL4796114 1 5.51
CHEMBL4783259 1 5.50
CHEMBL4781910 1 5.17
CHEMBL4779377 1 5.06
CHEMBL4794285 1 -
CHEMBL4788477 1 -
CHEMBL4781659 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1336 SORAFENIB IC50 = 9.0 nM 8.05
CHEMBL24828 VANDETANIB IC50 = 9.0 nM 8.05
CHEMBL4779887 IC50 = 10.0 nM 8.00
CHEMBL4793380 IC50 = 10.0 nM 8.00
CHEMBL4789515 IC50 = 10.0 nM 8.00
CHEMBL2105717 CABOZANTINIB IC50 = 13.0 nM 7.89
CHEMBL4784865 IC50 = 21.0 nM 7.68
CHEMBL4784000 IC50 = 22.0 nM 7.66
CHEMBL4797687 IC50 = 25.0 nM 7.60
CHEMBL4798046 IC50 = 50.0 nM 7.30
CHEMBL4794949 IC50 = 77.0 nM 7.11
CHEMBL4797171 IC50 = 177.0 nM 6.75
CHEMBL4793991 IC50 = 227.0 nM 6.64
CHEMBL4793949 IC50 = 306.0 nM 6.51
CHEMBL4786825 IC50 = 324.0 nM 6.49
CHEMBL4798842 IC50 = 322.0 nM 6.49
CHEMBL4789912 IC50 = 781.0 nM 6.11
CHEMBL4786580 IC50 = 865.0 nM 6.06
CHEMBL4798574 IC50 = 944.0 nM 6.03
CHEMBL4792943 IC50 = 1071.0 nM 5.97
CHEMBL4797671 IC50 = 1474.0 nM 5.83
CHEMBL4794893 IC50 = 2223.0 nM 5.65
CHEMBL4796114 IC50 = 3073.0 nM 5.51
CHEMBL4783259 IC50 = 3154.0 nM 5.50
CHEMBL4781910 IC50 = 6811.0 nM 5.17
CHEMBL4779377 IC50 = 8766.0 nM 5.06
CHEMBL4794285 IC50 > 10000.0 nM -
CHEMBL4788477 IC50 > 10000.0 nM -
CHEMBL4781659 IC50 > 10000.0 nM -