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Assay Detail

CHEMBL4767586

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NO711 from mouse GAT1 expressed in HEK293 cells measured after 40 mins of incubation by LC-ESI-MS-MS method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL5445)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Novel mouse GABA uptake inhibitors with enhanced inhibitory activity toward mGAT3/4 and their effect on pain threshold in mice.
Zaręba P,Gryzło B,Malawska K,Sałat K,Höfner GC,Nowaczyk A,Fijałkowski Ł,Rapacz A,Podkowa A,Furgała A,Żmudzki P,Wanner KT,Malawska B,Kulig K
Eur J Med Chem (2020) 188 : 111920 -111920
PubMed 31901745 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 4.88 8.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4595698 1 8.29
CHEMBL3289575 1 4.96
CHEMBL4780692 1 4.93
CHEMBL4796172 1 4.74
CHEMBL4795631 1 4.73
CHEMBL4785506 1 4.70
CHEMBL4798908 1 4.66
CHEMBL4792403 1 4.61
CHEMBL4780841 1 4.59
CHEMBL4791795 1 4.42
CHEMBL4777433 1 4.41
CHEMBL4787408 1 4.29
CHEMBL4779532 1 4.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4595698 Ki = 5.129 nM 8.29
CHEMBL3289575 Ki = 10964.78 nM 4.96
CHEMBL4780692 Ki = 11748.98 nM 4.93
CHEMBL4796172 Ki = 18197.01 nM 4.74
CHEMBL4795631 Ki = 18620.87 nM 4.73
CHEMBL4785506 Ki = 19952.62 nM 4.70
CHEMBL4798908 Ki = 21877.62 nM 4.66
CHEMBL4792403 Ki = 24547.09 nM 4.61
CHEMBL4780841 Ki = 25703.96 nM 4.59
CHEMBL4791795 Ki = 38018.94 nM 4.42
CHEMBL4777433 Ki = 38904.51 nM 4.41
CHEMBL4787408 Ki = 51286.14 nM 4.29
CHEMBL4779532 Ki = 87096.36 nM 4.06