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Assay Detail

CHEMBL4768583

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full-length N-terminal GST-fused CDK4 (1 to 303 residues)/GST-tagged CyclinD3 (1 to 292 residues) expressed in baculovirus expression system using ULight-elF4E-binding protein 1 peptide as substrate measured after 30 mins by LANCE assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK4/Cyclin D3 (CHEMBL3038472)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of a novel series of imidazo[1',2':1,6]pyrido[2,3-d]pyrimidin derivatives as potent cyclin-dependent kinase 4/6 inhibitors.
Shi C,Wang Q,Liao X,Ge H,Huo G,Zhang L,Chen N,Zhai X,Hong Y,Wang L,Wang Z,Shi W,Mao Y,Yu J,Ke Y,Xia G
Eur J Med Chem (2020) 193 : 112239 -112239
PubMed 32200202 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.98 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4778367 1 9.10
CHEMBL3301610 ABEMACICLIB 4.0 1 8.74
CHEMBL4792101 1 8.66
CHEMBL4794123 1 8.57
CHEMBL4776781 1 8.57
CHEMBL189963 PALBOCICLIB 4.0 1 8.49
CHEMBL4785811 1 8.44
CHEMBL4783539 1 8.43
CHEMBL4779845 1 8.40
CHEMBL4784940 1 8.38
CHEMBL4780277 1 8.35
CHEMBL4781009 1 8.33
CHEMBL4782575 1 8.28
CHEMBL4791259 1 8.00
CHEMBL4785079 1 8.00
CHEMBL4777368 1 7.99
CHEMBL4781277 1 7.87
CHEMBL4786042 1 7.72
CHEMBL4787909 1 7.65
CHEMBL4797327 1 7.58
CHEMBL4792839 1 7.23
CHEMBL4793422 1 7.00
CHEMBL4788066 1 6.91
CHEMBL4777250 1 6.57
CHEMBL4787501 1 6.23
CHEMBL4778835 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4778367 IC50 = 0.8 nM 9.10
CHEMBL3301610 ABEMACICLIB IC50 = 1.8 nM 8.74
CHEMBL4792101 IC50 = 2.2 nM 8.66
CHEMBL4794123 IC50 = 2.7 nM 8.57
CHEMBL4776781 IC50 = 2.7 nM 8.57
CHEMBL189963 PALBOCICLIB IC50 = 3.2 nM 8.49
CHEMBL4785811 IC50 = 3.6 nM 8.44
CHEMBL4783539 IC50 = 3.7 nM 8.43
CHEMBL4779845 IC50 = 4.0 nM 8.40
CHEMBL4784940 IC50 = 4.2 nM 8.38
CHEMBL4780277 IC50 = 4.5 nM 8.35
CHEMBL4781009 IC50 = 4.7 nM 8.33
CHEMBL4782575 IC50 = 5.3 nM 8.28
CHEMBL4791259 IC50 = 9.9 nM 8.00
CHEMBL4785079 IC50 = 9.9 nM 8.00
CHEMBL4777368 IC50 = 10.3 nM 7.99
CHEMBL4781277 IC50 = 13.5 nM 7.87
CHEMBL4786042 IC50 = 18.9 nM 7.72
CHEMBL4787909 IC50 = 22.3 nM 7.65
CHEMBL4797327 IC50 = 26.5 nM 7.58
CHEMBL4792839 IC50 = 59.0 nM 7.23
CHEMBL4793422 IC50 = 98.91 nM 7.00
CHEMBL4788066 IC50 = 123.0 nM 6.91
CHEMBL4777250 IC50 = 270.9 nM 6.57
CHEMBL4787501 IC50 = 592.3 nM 6.23
CHEMBL4778835 IC50 > 3333.0 nM -