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Assay Detail

CHEMBL4769103

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His6-tagged AMPK alpha1 using ULight CRBtide as substrate incubated for 1 hr followed by substrate addition and measured after 1 hr by TR-FRET assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Substituted oxindol-3-ylidenes as AMP-activated protein kinase (AMPK) inhibitors.
Matheson CJ,Casalvieri KA,Backos DS,Minhajuddin M,Jordan CT,Reigan P
Eur J Med Chem (2020) 197 : 112316 -112316
PubMed 32334266 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 6.66 7.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4794096 1 7.03
CHEMBL4781578 1 6.97
CHEMBL13976 1 6.87
CHEMBL4796639 1 6.86
CHEMBL4788980 1 6.82
CHEMBL535 SUNITINIB 4.0 1 6.80
CHEMBL4788376 1 6.79
CHEMBL4800500 1 6.76
CHEMBL3542344 1 6.75
CHEMBL4795330 1 6.74
CHEMBL4792246 1 6.69
CHEMBL4782796 1 6.68
CHEMBL4799580 1 6.66
CHEMBL4786972 1 6.66
CHEMBL4779166 1 6.62
CHEMBL275117 1 6.61
CHEMBL4781062 1 6.53
CHEMBL4785831 1 6.46
CHEMBL3542333 1 6.41
CHEMBL4799631 1 6.38
CHEMBL4779730 1 5.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4794096 IC50 = 93.0 nM 7.03
CHEMBL4781578 IC50 = 107.0 nM 6.97
CHEMBL13976 IC50 = 136.0 nM 6.87
CHEMBL4796639 IC50 = 139.0 nM 6.86
CHEMBL4788980 IC50 = 152.0 nM 6.82
CHEMBL535 SUNITINIB IC50 = 158.0 nM 6.80
CHEMBL4788376 IC50 = 164.0 nM 6.79
CHEMBL4800500 IC50 = 173.0 nM 6.76
CHEMBL3542344 IC50 = 180.0 nM 6.75
CHEMBL4795330 IC50 = 184.0 nM 6.74
CHEMBL4792246 IC50 = 205.0 nM 6.69
CHEMBL4782796 IC50 = 208.0 nM 6.68
CHEMBL4799580 IC50 = 221.0 nM 6.66
CHEMBL4786972 IC50 = 217.0 nM 6.66
CHEMBL4779166 IC50 = 240.0 nM 6.62
CHEMBL275117 IC50 = 246.0 nM 6.61
CHEMBL4781062 IC50 = 296.0 nM 6.53
CHEMBL4785831 IC50 = 348.0 nM 6.46
CHEMBL3542333 IC50 = 393.0 nM 6.41
CHEMBL4799631 IC50 = 418.0 nM 6.38
CHEMBL4779730 IC50 = 2100.0 nM 5.68