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Assay Detail

CHEMBL4770643

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I] alpha bungarotoxin from human neuronal alpha7 nAChR expressed in human SH-SY5Y cell membrane measured after 120 mins by Topcount scintillation counting method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-SY5Y
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor subunit alpha-7 (CHEMBL2492)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optical control of muscular nicotinic channels with azocuroniums, photoswitchable azobenzenes bearing two N-methyl-N-carbocyclic quaternary ammonium groups.
Herrera-Arozamena C,Estrada-Valencia M,Martí-Marí O,Pérez C,de la Fuente Revenga M,Villalba-Galea CA,Rodríguez-Franco MI
Eur J Med Chem (2020) 200 : 112403 -112403
PubMed 32447198 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 6.08 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL298826 (-)-EPIBATIDINE 1 6.92
CHEMBL4792178 1 6.14
CHEMBL4782657 1 6.04
CHEMBL4786777 1 6.03
CHEMBL4788647 1 5.72
CHEMBL4784491 1 5.60
CHEMBL4794082 1 -
CHEMBL4778999 1 -
CHEMBL3 NICOTINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL298826 (-)-EPIBATIDINE Ki = 120.0 nM 6.92
CHEMBL4792178 Ki = 730.0 nM 6.14
CHEMBL4782657 Ki = 910.0 nM 6.04
CHEMBL4786777 Ki = 930.0 nM 6.03
CHEMBL4788647 Ki = 1900.0 nM 5.72
CHEMBL4784491 Ki = 2500.0 nM 5.60
CHEMBL4794082 Ki > 10000.0 nM -
CHEMBL4778999 Ki > 10000.0 nM -
CHEMBL3 NICOTINE Ki - -