Assay Detail
Binding
CHEMBL4770645
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [125I] alpha bungarotoxin from human neuronal alpha4beta2 nAChR expressed in human SH-SY5Y cell membrane measured after 120 mins by Topcount scintillation counting method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | SH-SY5Y |
| Subcellular | Membrane |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Optical control of muscular nicotinic channels with azocuroniums, photoswitchable azobenzenes bearing two N-methyl-N-carbocyclic quaternary ammonium groups.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 9 | 7.32 | 8.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3 | NICOTINE | 4.0 | 1 | 8.82 |
| CHEMBL4794082 | — | — | 1 | 5.82 |
| CHEMBL4784491 | — | — | 1 | - |
| CHEMBL4786777 | — | — | 1 | - |
| CHEMBL4782657 | — | — | 1 | - |
| CHEMBL4788647 | — | — | 1 | - |
| CHEMBL4792178 | — | — | 1 | - |
| CHEMBL4778999 | — | — | 1 | - |
| CHEMBL298826 | (-)-EPIBATIDINE | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3 | NICOTINE | Ki | = | 1.5 | nM | 8.82 |
| CHEMBL4794082 | — | Ki | = | 1500.0 | nM | 5.82 |
| CHEMBL4784491 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL4786777 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL4782657 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL4788647 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL4792178 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL4778999 | — | Ki | > | 10000.0 | nM | - |
| CHEMBL298826 | (-)-EPIBATIDINE | Ki | - | — | - |