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Assay Detail

CHEMBL4770962

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant HDAC8 using H2N-Arg-His-Lys(Ac)-Lys(Ac)-AMC as substrate after 90 mins by fluorescence based micro plate assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 8 (CHEMBL3192)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological evaluation of dual targeting inhibitors of histone deacetylase 6/8 and bromodomain BRPF1.
Ghazy E,Zeyen P,Herp D,Hügle M,Schmidtkunz K,Erdmann F,Robaa D,Schmidt M,Morales ER,Romier C,Günther S,Jung M,Sippl W
Eur J Med Chem (2020) 200 : 112338 -112338
PubMed 32497960 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.74 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4534486 1 9.10
CHEMBL4078458 1 7.57
CHEMBL4206936 1 7.30
CHEMBL4789607 1 7.19
CHEMBL4105068 1 7.16
CHEMBL4790363 1 6.95
CHEMBL4781368 1 6.80
CHEMBL4784767 1 6.64
CHEMBL4788940 1 6.44
CHEMBL4786813 1 6.35
CHEMBL4786752 1 6.33
CHEMBL4780727 1 6.26
CHEMBL4782406 1 6.25
CHEMBL4789481 1 6.04
CHEMBL4792281 1 6.02
CHEMBL4797308 1 5.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4534486 IC50 = 0.8 nM 9.10
CHEMBL4078458 IC50 = 27.0 nM 7.57
CHEMBL4206936 IC50 = 50.0 nM 7.30
CHEMBL4789607 IC50 = 65.0 nM 7.19
CHEMBL4105068 IC50 = 70.0 nM 7.16
CHEMBL4790363 IC50 = 113.0 nM 6.95
CHEMBL4781368 IC50 = 158.0 nM 6.80
CHEMBL4784767 IC50 = 231.0 nM 6.64
CHEMBL4788940 IC50 = 360.0 nM 6.44
CHEMBL4786813 IC50 = 443.0 nM 6.35
CHEMBL4786752 IC50 = 465.0 nM 6.33
CHEMBL4780727 IC50 = 555.0 nM 6.26
CHEMBL4782406 IC50 = 560.0 nM 6.25
CHEMBL4789481 IC50 = 908.0 nM 6.04
CHEMBL4792281 IC50 = 956.0 nM 6.02
CHEMBL4797308 IC50 = 3193.0 nM 5.50