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Assay Detail

CHEMBL4771496

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full length recombinant HDAC6 using p53 (379 to 382 residues) (RHKK(Ac)AMC) as substrate preincubated for 5 to 10 mins followed by substrate addition and measured after 2 hrs by fluorescence method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Thirty Years of HDAC Inhibitors: 2020 Insight and Hindsight.
Ho TCS,Chan AHY,Ganesan A
J Med Chem (2020) 63 : 12460 -12484
PubMed 32608981 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.55 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4536605 1 8.70
CHEMBL2018302 TUBASTATIN A 1 8.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4536605 IC50 = 2.0 nM 8.70
CHEMBL2018302 TUBASTATIN A IC50 = 4.0 nM 8.40