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Assay Detail

CHEMBL4772279

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of TAMRA-NPFF from human NPFFR2 expressed in CHO-K1 cells preincubated for 15 mins followed by GTPgammaS addition and measured after 15 to 120 mins by competitive binding assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuropeptide FF receptor 2 (CHEMBL5952)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Neuropeptide FF and Its Receptors: Therapeutic Applications and Ligand Development.
Nguyen T,Marusich J,Li JX,Zhang Y
J Med Chem (2020) 63 : 12387 -12402
PubMed 32673481 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 8.76 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4779743 1 8.85
CHEMBL4800299 1 8.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4779743 Ki = 1.42 nM 8.85
CHEMBL4800299 Ki = 2.17 nM 8.66