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Assay Detail

CHEMBL4773458

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at CXCR4 in human CD4-positive T cells assessed as inhibition of CXCL12-induced cytosolic calcium flux preincubated for 20 mins followed by CXCL12 addition by calcium 4 dye based FLIPR TETRA analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type T cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-X-C chemokine receptor type 4 (CHEMBL2107)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and evaluation of pyrrolidine based CXCR4 antagonists with in vivo anti-tumor metastatic activity.
Li Z,Wang X,Lin Y,Wang Y,Wu S,Xia K,Xu C,Ma H,Zheng J,Luo L,Zhu F,He S,Zhang X
Eur J Med Chem (2020) 205 : 112537 -112537
PubMed 32768738 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 8.69 9.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4799439 1 9.60
CHEMBL4792975 1 9.40
CHEMBL4799191 1 8.96
CHEMBL4777386 1 8.74
CHEMBL4783831 1 8.57
CHEMBL4789233 1 8.57
CHEMBL4795444 1 8.31
CHEMBL18442 PLERIXAFOR 4.0 1 8.21
CHEMBL4787045 1 7.89
CHEMBL4797343 1 -
CHEMBL4783241 1 -
CHEMBL4785166 1 -
CHEMBL4777987 1 -
CHEMBL4794732 1 -
CHEMBL4795219 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4799439 IC50 = 0.25 nM 9.60
CHEMBL4792975 IC50 = 0.4 nM 9.40
CHEMBL4799191 IC50 = 1.1 nM 8.96
CHEMBL4777386 IC50 = 1.8 nM 8.74
CHEMBL4783831 IC50 = 2.7 nM 8.57
CHEMBL4789233 IC50 = 2.7 nM 8.57
CHEMBL4795444 IC50 = 4.9 nM 8.31
CHEMBL18442 PLERIXAFOR IC50 = 6.134 nM 8.21
CHEMBL4787045 IC50 = 13.0 nM 7.89
CHEMBL4797343 IC50 - -
CHEMBL4783241 IC50 - -
CHEMBL4785166 IC50 - -
CHEMBL4777987 IC50 - -
CHEMBL4794732 IC50 - -
CHEMBL4795219 IC50 - -