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Assay Detail

CHEMBL4773797

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS CoV2 C-terminal His6-tagged 3CL protease expressed in Escherichia coli BL21 DE3 using Thr-Ser-Ala-Val-Leu-Gln-pNA as substrate preincubated for 30 mins followed by substrate addition and measured for 20 mins by colorimetric method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Potent inhibitors of SARS-CoV-2 3C-like protease derived from N-substituted isatin compounds.
Liu P,Liu H,Sun Q,Liang H,Li C,Deng X,Liu Y,Lai L
Eur J Med Chem (2020) 206 : 112702 -112702
PubMed 32798789 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.56 7.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL358279 1 7.35
CHEMBL4784404 1 7.33
CHEMBL149950 1 7.28
CHEMBL3545157 TIDEGLUSIB 2.0 1 5.72
CHEMBL210525 1 4.99
CHEMBL4791652 1 4.81
CHEMBL379727 1 4.75
CHEMBL4795325 1 4.60
CHEMBL4778529 1 4.41
CHEMBL209287 1 4.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL358279 IC50 = 45.0 nM 7.35
CHEMBL4784404 IC50 = 47.0 nM 7.33
CHEMBL149950 IC50 = 53.0 nM 7.28
CHEMBL3545157 TIDEGLUSIB IC50 = 1910.0 nM 5.72
CHEMBL210525 IC50 = 10200.0 nM 4.99
CHEMBL4791652 IC50 = 15500.0 nM 4.81
CHEMBL379727 IC50 = 17800.0 nM 4.75
CHEMBL4795325 IC50 = 24900.0 nM 4.60
CHEMBL4778529 IC50 = 39200.0 nM 4.41
CHEMBL209287 IC50 = 41800.0 nM 4.38