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Assay Detail

CHEMBL4775971

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to His6-tagged Escherichia coli FabF C163Q mutant expressed in Escherichia coli BL21 (DE3) assessed as binding constant measured after 200 sec by isothermal titration calorimetry
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Semisynthesis and Biological Evaluation of Platencin Thioether Derivatives: Dual FabF and FabH Inhibitors against MRSA.
Li Y,Weng X,Deng Y,Pan J,Zhu S,Wen Z,Yuan Y,Li S,Shen B,Duan Y,Huang Y
ACS Med Chem Lett (2021) 12 : 433 -442
PubMed 33738071 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 7.24 7.76

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1092943 PLATENCIN 1 7.76
CHEMBL411278 1 7.57
CHEMBL4800275 1 6.99
CHEMBL4793993 1 6.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1092943 PLATENCIN Kd = 17.28 nM 7.76
CHEMBL411278 Kd = 26.97 nM 7.57
CHEMBL4800275 Kd = 101.7 nM 6.99
CHEMBL4793993 Kd = 223.2 nM 6.65