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Assay Detail

CHEMBL4810446

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to FLAG-tagged GRK6 in human KMS11 cells incubated for 2.5 hrs by ITDR assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type KMS-11
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G protein-coupled receptor kinase 6 (CHEMBL6144)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Characterization of 4-Aminoquinazolines as Potent Inhibitors of the G Protein-Coupled Receptor Kinase 6 (GRK6) for the Treatment of Multiple Myeloma.
Uehling DE, Joseph B, Chung KC, Zhang AX, Ler S, Prakesch MA, Poda G, Grouleff J, Aman A, Kiyota T, Leung-Hagesteijn C, Konda JD, Marcellus R, Griffin C, Subramaniam R, Abibi A, Strathdee CA, Isaac MB, Al-Awar R, Tiedemann RE.
J Med Chem (2021) 64 : 11129 -11147
PubMed 34291633 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.15 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4877302 1 7.52
CHEMBL4860332 1 6.77
CHEMBL4875929 1 6.21
CHEMBL4854871 1 5.70
CHEMBL4874424 1 5.70
CHEMBL4847703 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4877302 IC50 = 30.0 nM 7.52
CHEMBL4860332 IC50 = 170.0 nM 6.77
CHEMBL4875929 IC50 = 610.0 nM 6.21
CHEMBL4854871 IC50 = 2000.0 nM 5.70
CHEMBL4874424 IC50 = 2000.0 nM 5.70
CHEMBL4847703 IC50 = 10000.0 nM 5.00