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Assay Detail

CHEMBL4810465

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Binding
Binding affinity to Burkholderia pseudomallei IspF at pH 7.4 measured by isothermal titration calorimetry method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Burkholderia pseudomallei
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (CHEMBL3091270)
Type SINGLE PROTEIN
Organism Burkholderia pseudomallei (strain K96243)

Publication

Structural and biophysical characterization of the Burkholderia pseudomallei IspF inhibitor L-tryptophan hydroxamate.
Blain JM, Grote DL, Watkins SM, Goshu GM, Muller C, Gorman JL, Ranieri G, Walter RL, Hofstetter H, Horn JR, Hagen TJ.
Bioorg Med Chem Lett (2021) 48 : 128273 -128273
PubMed 34298132 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 4.32 4.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL490034 1 4.44
CHEMBL4874588 1 4.19
CHEMBL54976 TRYPTOPHAN 4.0 1 -
CHEMBL292303 D-TRYPTOPHAN 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL490034 Kd = 36000.0 nM 4.44
CHEMBL4874588 Kd = 64000.0 nM 4.19
CHEMBL54976 TRYPTOPHAN Kd > 1000000.0 nM -
CHEMBL292303 D-TRYPTOPHAN Kd > 1000000.0 nM -