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Assay Detail

CHEMBL4811595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged full length human recombinant HDAC6 expressed in baculovirus infected Sf9 cells using ZMAL (Z-Lys(Ac)-AMC) fluorogenic substrate incubated for 90 mins by fluorescence based assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Investigation of the in vitro and in vivo efficacy of peptoid-based HDAC inhibitors with dual-stage antiplasmodial activity.
Mackwitz MKW, Hesping E, Eribez K, Schöler A, Antonova-Koch Y, Held J, Winzeler EA, Andrews KT, Hansen FK.
Eur J Med Chem (2021) 211 : 113065 -113065
PubMed 33360801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.27 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4854577 1 8.00
CHEMBL2179618 NEXTURASTAT A 1 7.70
CHEMBL4873872 1 7.70
CHEMBL4854335 1 7.52
CHEMBL4851776 1 7.52
CHEMBL4847978 1 7.52
CHEMBL4853947 1 7.40
CHEMBL4873978 1 7.40
CHEMBL98 VORINOSTAT 4.0 1 7.30
CHEMBL4849235 1 7.30
CHEMBL4868526 1 7.22
CHEMBL4878499 1 7.22
CHEMBL4849568 1 7.22
CHEMBL4866446 1 7.16
CHEMBL4878538 1 6.89
CHEMBL4868592 1 6.64
CHEMBL4862927 1 6.60
CHEMBL4858841 1 6.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4854577 IC50 = 10.0 nM 8.00
CHEMBL2179618 NEXTURASTAT A IC50 = 20.0 nM 7.70
CHEMBL4873872 IC50 = 20.0 nM 7.70
CHEMBL4854335 IC50 = 30.0 nM 7.52
CHEMBL4851776 IC50 = 30.0 nM 7.52
CHEMBL4847978 IC50 = 30.0 nM 7.52
CHEMBL4853947 IC50 = 40.0 nM 7.40
CHEMBL4873978 IC50 = 40.0 nM 7.40
CHEMBL98 VORINOSTAT IC50 = 50.0 nM 7.30
CHEMBL4849235 IC50 = 50.0 nM 7.30
CHEMBL4868526 IC50 = 60.0 nM 7.22
CHEMBL4878499 IC50 = 60.0 nM 7.22
CHEMBL4849568 IC50 = 60.0 nM 7.22
CHEMBL4866446 IC50 = 70.0 nM 7.16
CHEMBL4878538 IC50 = 130.0 nM 6.89
CHEMBL4868592 IC50 = 230.0 nM 6.64
CHEMBL4862927 IC50 = 250.0 nM 6.60
CHEMBL4858841 IC50 = 270.0 nM 6.57