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Assay Detail

CHEMBL4811769

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Immunosuppressive activity in C57BL/6J mouse CD4+ve T cells assessed as inhibition of CD3/CD28-stimulated CD4+ve T cell proliferation incubated for 72 hrs by CellTrace-violet staining based flow cytometry analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CD4+ve Th
Confidence 1 — Organism
Curated By Autocuration

Target

CD4+ve Th (CHEMBL613544)
Type CELL-LINE

Publication

Biosynthesis of Nonimmunosuppressive ProlylFK506 Analogues with Neurite Outgrowth and Synaptogenic Activity.
Jung JA, Lee HJ, Song MC, Hwangbo A, Beom JY, Lee SJ, Park DJ, Oh JH, Ha SJ, Cheong E, Yoon YJ.
J Nat Prod (2021) 84 : 195 -203
PubMed 33534559 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.00 10.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL269732 TACROLIMUS ANHYDROUS 4.0 1 10.47
CHEMBL4877265 1 5.40
CHEMBL4876168 1 5.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL269732 TACROLIMUS ANHYDROUS IC50 = 0.034 nM 10.47
CHEMBL4877265 IC50 = 3969.0 nM 5.40
CHEMBL4876168 IC50 = 7273.0 nM 5.14