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Assay Detail

CHEMBL4812908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Trypanosoma brucei rhodesain using Z-FR-AMC as substrate by fluorimetric analysis relative to control
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma brucei
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Rhodesain (CHEMBL4188)
Type SINGLE PROTEIN
Organism Trypanosoma brucei rhodesiense

Publication

Computer-aided design of 1,4-naphthoquinone-based inhibitors targeting cruzain and rhodesain cysteine proteases.
Silva LR, Guimarães AS, do Nascimento J, do Santos Nascimento IJ, da Silva EB, McKerrow JH, Cardoso SH, da Silva-Júnior EF.
Bioorg Med Chem (2021) 41 : 116213 -116213
PubMed 33992862 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.41 4.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4845877 1 4.54
CHEMBL4877896 1 4.52
CHEMBL4859457 1 4.18
CHEMBL240963 LAWSONE 1 -
CHEMBL15193 LAPACHOL 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4845877 IC50 = 29000.0 nM 4.54
CHEMBL4877896 IC50 = 30000.0 nM 4.52
CHEMBL4859457 IC50 = 66000.0 nM 4.18
CHEMBL240963 LAWSONE IC50 > 100000.0 nM -
CHEMBL15193 LAPACHOL IC50 > 100000.0 nM -