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Assay Detail

CHEMBL4817020

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Anti-necroptotic activity in human HT-29 cells assessed as inhibition of TNFalpha/Z-VAD-fmk (TSZ)-induced necroptosis by measuring increase in cell viability by celltiter-glo luminescent cell viability assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Ring closure strategy leads to potent RIPK3 inhibitors.
Wu S, Xu C, Xia K, Lin Y, Tian S, Ma H, Ji Y, Zhu F, He S, Zhang X.
Eur J Med Chem (2021) 217 : 113327 -113327
PubMed 33730678 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 19 5.73 6.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4861646 1 6.38
CHEMBL4877986 1 6.37
CHEMBL4877249 1 5.99
CHEMBL4862270 1 5.99
CHEMBL4876332 1 5.97
CHEMBL4779280 1 5.82
CHEMBL4866260 1 5.66
CHEMBL4854299 1 5.65
CHEMBL4846511 1 5.64
CHEMBL4877658 1 5.42
CHEMBL4852815 1 5.40
CHEMBL4879079 1 4.48
CHEMBL4853076 1 -
CHEMBL4868642 1 -
CHEMBL4870302 1 -
CHEMBL4870972 1 -
CHEMBL4867075 1 -
CHEMBL4857598 1 -
CHEMBL4858109 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4861646 EC50 = 420.0 nM 6.38
CHEMBL4877986 EC50 = 430.0 nM 6.37
CHEMBL4877249 EC50 = 1030.0 nM 5.99
CHEMBL4862270 EC50 = 1030.0 nM 5.99
CHEMBL4876332 EC50 = 1070.0 nM 5.97
CHEMBL4779280 EC50 = 1510.0 nM 5.82
CHEMBL4866260 EC50 = 2190.0 nM 5.66
CHEMBL4854299 EC50 = 2230.0 nM 5.65
CHEMBL4846511 EC50 = 2280.0 nM 5.64
CHEMBL4877658 EC50 = 3810.0 nM 5.42
CHEMBL4852815 EC50 = 3960.0 nM 5.40
CHEMBL4879079 EC50 = 33000.0 nM 4.48
CHEMBL4853076 EC50 > 20000.0 nM -
CHEMBL4868642 EC50 > 20000.0 nM -
CHEMBL4870302 EC50 > 20000.0 nM -
CHEMBL4870972 EC50 > 20000.0 nM -
CHEMBL4867075 EC50 > 20000.0 nM -
CHEMBL4857598 EC50 > 20000.0 nM -
CHEMBL4858109 EC50 > 20000.0 nM -