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Assay Detail

CHEMBL4817528

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Binding
Inhibition of ASM in dog MDCK cells co-expressing recombinant full length mGFP-tagged KRAS G12V mutant and mCherry-CAAX assessed as reduction in plasma membrane localization of KRAS mutant incubated for 48 hrs by confocal microscopic analysis
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Canis lupus familiaris
Cell Type MDCK
Subcellular Plasma Membrane
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Scaffold repurposing of fendiline: Identification of potent KRAS plasma membrane localization inhibitors.
Wang P, van der Hoeven D, Ye N, Chen H, Liu Z, Ma X, Montufar-Solis D, Rehl KM, Cho KJ, Thapa S, Chen W, van der Hoeven R, Frost JA, Hancock JF, Zhou J.
Eur J Med Chem (2021) 217 : 113381 -113381
PubMed 33756124 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 5.68 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4854753 1 7.70
CHEMBL4858481 1 7.00
CHEMBL4878364 1 6.70
CHEMBL4859726 1 6.52
CHEMBL4849536 1 6.30
CHEMBL4868505 1 6.30
CHEMBL4871394 1 6.22
CHEMBL4859822 1 6.22
CHEMBL4876631 1 5.96
CHEMBL4878842 1 5.92
CHEMBL4851942 1 5.92
CHEMBL4847178 1 5.89
CHEMBL4862403 1 5.82
CHEMBL4864189 1 5.55
CHEMBL4871910 1 5.48
CHEMBL4870504 1 5.42
CHEMBL4858212 1 5.42
CHEMBL4862663 1 5.36
CHEMBL4851435 1 5.28
CHEMBL4871115 1 5.15
CHEMBL4861239 1 5.11
CHEMBL4850799 1 5.00
CHEMBL4846949 1 4.97
CHEMBL4867468 1 4.95
CHEMBL4875085 1 4.95
CHEMBL4854899 1 4.89
CHEMBL4845855 1 4.83
CHEMBL4867441 1 4.23
CHEMBL4860440 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4854753 IC50 = 20.0 nM 7.70
CHEMBL4858481 IC50 = 100.0 nM 7.00
CHEMBL4878364 IC50 = 200.0 nM 6.70
CHEMBL4859726 IC50 = 300.0 nM 6.52
CHEMBL4849536 IC50 = 500.0 nM 6.30
CHEMBL4868505 IC50 = 500.0 nM 6.30
CHEMBL4871394 IC50 = 600.0 nM 6.22
CHEMBL4859822 IC50 = 600.0 nM 6.22
CHEMBL4876631 IC50 = 1100.0 nM 5.96
CHEMBL4878842 IC50 = 1200.0 nM 5.92
CHEMBL4851942 IC50 = 1200.0 nM 5.92
CHEMBL4847178 IC50 = 1300.0 nM 5.89
CHEMBL4862403 IC50 = 1500.0 nM 5.82
CHEMBL4864189 IC50 = 2800.0 nM 5.55
CHEMBL4871910 IC50 = 3300.0 nM 5.48
CHEMBL4870504 IC50 = 3800.0 nM 5.42
CHEMBL4858212 IC50 = 3800.0 nM 5.42
CHEMBL4862663 IC50 = 4400.0 nM 5.36
CHEMBL4851435 IC50 = 5200.0 nM 5.28
CHEMBL4871115 IC50 = 7100.0 nM 5.15
CHEMBL4861239 IC50 = 7700.0 nM 5.11
CHEMBL4850799 IC50 = 10100.0 nM 5.00
CHEMBL4846949 IC50 = 10600.0 nM 4.97
CHEMBL4867468 IC50 = 11200.0 nM 4.95
CHEMBL4875085 IC50 = 11200.0 nM 4.95
CHEMBL4854899 IC50 = 13000.0 nM 4.89
CHEMBL4845855 IC50 = 14800.0 nM 4.83
CHEMBL4867441 IC50 = 59300.0 nM 4.23
CHEMBL4860440 IC50 - -