Assay Detail
Binding
CHEMBL4818541
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Inhibition of recombinant human C-terminal FLAG-tagged HDAC1 expressed in human HEK293F cells using Fluor-de-lys substrate as substrate incubated for 3 hrs followed by substrate addition and measured after 60 mins by fluorescence based assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293F |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Redefining the Histone Deacetylase Inhibitor Pharmacophore: High Potency with No Zinc Cofactor Interaction.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 6.70 | 9.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4873847 | — | — | 1 | 9.00 |
| CHEMBL2448576 | — | — | 1 | 8.70 |
| CHEMBL4867665 | — | — | 1 | 8.70 |
| CHEMBL4879154 | — | — | 1 | 7.80 |
| CHEMBL4861270 | — | — | 1 | 7.43 |
| CHEMBL4872186 | — | — | 1 | 7.33 |
| CHEMBL4858081 | — | — | 1 | 6.60 |
| CHEMBL4851511 | — | — | 1 | 6.55 |
| CHEMBL4862719 | — | — | 1 | 6.41 |
| CHEMBL4874919 | — | — | 1 | 6.11 |
| CHEMBL4877454 | — | — | 1 | 5.33 |
| CHEMBL4878011 | — | — | 1 | 4.75 |
| CHEMBL4859130 | — | — | 1 | 4.64 |
| CHEMBL4855240 | — | — | 1 | 4.39 |
| CHEMBL4851062 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4873847 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL2448576 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL4867665 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL4879154 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL4861270 | — | IC50 | = | 37.0 | nM | 7.43 |
| CHEMBL4872186 | — | IC50 | = | 47.0 | nM | 7.33 |
| CHEMBL4858081 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL4851511 | — | IC50 | = | 280.0 | nM | 6.55 |
| CHEMBL4862719 | — | IC50 | = | 390.0 | nM | 6.41 |
| CHEMBL4874919 | — | IC50 | = | 770.0 | nM | 6.11 |
| CHEMBL4877454 | — | IC50 | = | 4700.0 | nM | 5.33 |
| CHEMBL4878011 | — | IC50 | = | 18000.0 | nM | 4.75 |
| CHEMBL4859130 | — | IC50 | = | 23000.0 | nM | 4.64 |
| CHEMBL4855240 | — | IC50 | = | 41000.0 | nM | 4.39 |
| CHEMBL4851062 | — | IC50 | > | 50000.0 | nM | - |