Assay Detail
Binding
CHEMBL4818542
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Inhibition of recombinant human C-terminal FLAG-tagged HDAC2 expressed in human HEK293F cells using Fluor-de-lys substrate as substrate incubated for 3 hrs followed by substrate addition and measured after 60 mins by fluorescence based assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293F |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Redefining the Histone Deacetylase Inhibitor Pharmacophore: High Potency with No Zinc Cofactor Interaction.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 6.93 | 8.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4867665 | — | — | 1 | 8.30 |
| CHEMBL2448576 | — | — | 1 | 8.15 |
| CHEMBL4873847 | — | — | 1 | 8.05 |
| CHEMBL4879154 | — | — | 1 | 7.39 |
| CHEMBL4861270 | — | — | 1 | 7.06 |
| CHEMBL4872186 | — | — | 1 | 6.92 |
| CHEMBL4858081 | — | — | 1 | 6.30 |
| CHEMBL4851511 | — | — | 1 | 6.12 |
| CHEMBL4874919 | — | — | 1 | 5.57 |
| CHEMBL4862719 | — | — | 1 | 5.47 |
| CHEMBL4878011 | — | — | 1 | - |
| CHEMBL4877454 | — | — | 1 | - |
| CHEMBL4859130 | — | — | 1 | - |
| CHEMBL4851062 | — | — | 1 | - |
| CHEMBL4855240 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4867665 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL2448576 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL4873847 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL4879154 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL4861270 | — | IC50 | = | 87.0 | nM | 7.06 |
| CHEMBL4872186 | — | IC50 | = | 120.0 | nM | 6.92 |
| CHEMBL4858081 | — | IC50 | = | 500.0 | nM | 6.30 |
| CHEMBL4851511 | — | IC50 | = | 750.0 | nM | 6.12 |
| CHEMBL4874919 | — | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL4862719 | — | IC50 | = | 3400.0 | nM | 5.47 |
| CHEMBL4878011 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4877454 | — | IC50 | > | 17000.0 | nM | - |
| CHEMBL4859130 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4851062 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4855240 | — | IC50 | > | 50000.0 | nM | - |