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Assay Detail

CHEMBL4818550

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of class 1 HDAC in human Jurkat 2C4 model of HIV latency assessed as reactivation of HIV latency incubated for 18 to 24 hrs in presence of 5% heat inactivated NHS by Steady-Glo luciferase assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type 2C4
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Class 1 histone deacetylase (CHEMBL4523988)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Redefining the Histone Deacetylase Inhibitor Pharmacophore: High Potency with No Zinc Cofactor Interaction.
Beshore DC, Adam GC, Barnard RJO, Burlein C, Gallicchio SN, Holloway MK, Krosky D, Lemaire W, Myers RW, Patel S, Plotkin MA, Powell DA, Rada V, Cox CD, Coleman PJ, Klein DJ, Wolkenberg SE.
ACS Med Chem Lett (2021) 12 : 540 -547
PubMed 33854701 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.73 6.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4873847 1 6.23
CHEMBL408513 BELINOSTAT 4.0 1 6.21
CHEMBL2448576 1 6.00
CHEMBL98 VORINOSTAT 4.0 1 5.72
CHEMBL4861270 1 5.50
CHEMBL4879154 1 5.32
CHEMBL4867665 1 5.13

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4873847 IC50 = 590.0 nM 6.23
CHEMBL408513 BELINOSTAT IC50 = 620.0 nM 6.21
CHEMBL2448576 IC50 = 1000.0 nM 6.00
CHEMBL98 VORINOSTAT IC50 = 1900.0 nM 5.72
CHEMBL4861270 IC50 = 3200.0 nM 5.50
CHEMBL4879154 IC50 = 4800.0 nM 5.32
CHEMBL4867665 IC50 = 7400.0 nM 5.13