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Assay Detail

CHEMBL4820883

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human glycine receptor subunit alpha-1 expressed in HEK293 cells assessed as reduction in glycine-induced currents by whole-cell patch-clamp assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycine receptor subunit alpha-1 (CHEMBL5845)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

C-2-Linked Dimeric Strychnine Analogues as Bivalent Ligands Targeting Glycine Receptors.
Zlotos DP, Abdelmalek CM, Botros LS, Banoub MM, Mandour YM, Breitinger U, El Nady A, Breitinger HG, Sotriffer C, Villmann C, Jensen AA, Holzgrabe U.
J Nat Prod (2021) 84 : 382 -394
PubMed 33596384 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.31 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL227934 STRYCHNINE -1.0 1 7.30
CHEMBL4849489 1 7.05
CHEMBL4870499 1 7.00
CHEMBL4857611 1 6.89
CHEMBL4851242 1 6.77
CHEMBL4848356 1 6.40
CHEMBL4850957 1 6.14
CHEMBL4860026 1 5.96
CHEMBL4873219 1 5.85
CHEMBL4849060 1 5.82
CHEMBL4849579 1 5.77
CHEMBL4861263 1 5.54
CHEMBL4878671 1 5.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL227934 STRYCHNINE IC50 = 50.0 nM 7.30
CHEMBL4849489 IC50 = 90.0 nM 7.05
CHEMBL4870499 IC50 = 100.0 nM 7.00
CHEMBL4857611 IC50 = 130.0 nM 6.89
CHEMBL4851242 IC50 = 170.0 nM 6.77
CHEMBL4848356 IC50 = 400.0 nM 6.40
CHEMBL4850957 IC50 = 720.0 nM 6.14
CHEMBL4860026 IC50 = 1100.0 nM 5.96
CHEMBL4873219 IC50 = 1400.0 nM 5.85
CHEMBL4849060 IC50 = 1500.0 nM 5.82
CHEMBL4849579 IC50 = 1700.0 nM 5.77
CHEMBL4861263 IC50 = 2900.0 nM 5.54
CHEMBL4878671 IC50 = 3100.0 nM 5.51