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Assay Detail

CHEMBL4821172

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BACE1 (1 to 454 residues) (unknown origin) using APP harboring Swedish Lys/Met mutant-derived peptide as substrate by FRET assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Approaches to Improving Selectivity through Utilizing Explicit Water Molecules: Discovery of Selective β-Secretase (BACE1) Inhibitors over BACE2.
Fujimoto K, Yoshida S, Tadano G, Asada N, Fuchino K, Suzuki S, Matsuoka E, Yamamoto T, Yamamoto S, Ando S, Kanegawa N, Tonomura Y, Ito H, Moechars D, Rombouts FJR, Gijsen HJM, Kusakabe KI.
J Med Chem (2021) 64 : 3075 -3085
PubMed 33719429 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.61 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3301601 VERUBECESTAT 3.0 1 8.72
CHEMBL4864591 1 7.82
CHEMBL4854629 1 7.62
CHEMBL2347203 1 7.50
CHEMBL4852444 1 7.15
CHEMBL4856391 1 6.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3301601 VERUBECESTAT IC50 = 1.9 nM 8.72
CHEMBL4864591 IC50 = 15.0 nM 7.82
CHEMBL4854629 IC50 = 24.0 nM 7.62
CHEMBL2347203 IC50 = 32.0 nM 7.50
CHEMBL4852444 IC50 = 71.0 nM 7.15
CHEMBL4856391 IC50 = 145.0 nM 6.84