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Assay Detail

CHEMBL4821429

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human BACE1 expressed in baculovirus expression system using Rh-EVNLDAEFK-quencher as substrate incubated for 60 mins by FRET assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of multifunctional anti-Alzheimer's agents with a unique mechanism of action including inhibition of the enzyme butyrylcholinesterase and γ-aminobutyric acid transporters.
Pasieka A, Panek D, Jończyk J, Godyń J, Szałaj N, Latacz G, Tabor J, Mezeiova E, Chantegreil F, Dias J, Knez D, Lu J, Pi R, Korabecny J, Brazzolotto X, Gobec S, Höfner G, Wanner K, Więckowska A, Malawska B.
Eur J Med Chem (2021) 218 : 113397 -113397
PubMed 33838585 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.14 7.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL378225 1 7.34
CHEMBL4857933 1 5.68
CHEMBL4868077 1 5.47
CHEMBL4876288 1 5.34
CHEMBL4874266 1 5.23
CHEMBL4858894 1 5.22
CHEMBL4851448 1 5.21
CHEMBL4861746 1 5.18
CHEMBL4877574 1 5.08
CHEMBL4856694 1 5.07
CHEMBL4873410 1 5.05
CHEMBL4857355 1 4.81
CHEMBL4845832 1 4.76
CHEMBL4847981 1 4.63
CHEMBL4860617 1 4.47
CHEMBL4869613 1 4.45
CHEMBL4860825 1 4.38
CHEMBL502 DONEPEZIL 4.0 1 -
CHEMBL95 TACRINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL378225 IC50 = 46.0 nM 7.34
CHEMBL4857933 IC50 = 2100.0 nM 5.68
CHEMBL4868077 IC50 = 3400.0 nM 5.47
CHEMBL4876288 IC50 = 4600.0 nM 5.34
CHEMBL4874266 IC50 = 5900.0 nM 5.23
CHEMBL4858894 IC50 = 6000.0 nM 5.22
CHEMBL4851448 IC50 = 6200.0 nM 5.21
CHEMBL4861746 IC50 = 6600.0 nM 5.18
CHEMBL4877574 IC50 = 8300.0 nM 5.08
CHEMBL4856694 IC50 = 8600.0 nM 5.07
CHEMBL4873410 IC50 = 8900.0 nM 5.05
CHEMBL4857355 IC50 = 15400.0 nM 4.81
CHEMBL4845832 IC50 = 17400.0 nM 4.76
CHEMBL4847981 IC50 = 23400.0 nM 4.63
CHEMBL4860617 IC50 = 33600.0 nM 4.47
CHEMBL4869613 IC50 = 35400.0 nM 4.45
CHEMBL4860825 IC50 = 41600.0 nM 4.38
CHEMBL502 DONEPEZIL IC50 - -
CHEMBL95 TACRINE IC50 - -