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Assay Detail

CHEMBL4821489

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of C-terminal His-tagged human HDAC9 (604 to 1066 residues) expressed in baculovirus-infected Sf9 cells using Boc Lys(TFA)-AMC as substrate preincubated for 5 mins followed by substrate addition and further incubated for 30 mins by fluorogenic assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 9 (CHEMBL4145)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of phenothiazine derivative-containing hydroxamic acids as potent class II histone deacetylase inhibitors.
Hsu KC, Chu JC, Tseng HJ, Liu CI, Wang HC, Lin TE, Lee HS, Hsin LW, Wang AH, Lin CH, Huang WJ.
Eur J Med Chem (2021) 219 : 113419 -113419
PubMed 33845233 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.47 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4530950 1 6.46
CHEMBL4862510 1 6.23
CHEMBL4872697 1 6.14
CHEMBL4852441 1 6.14
CHEMBL4874716 1 6.07
CHEMBL4861336 1 6.05
CHEMBL4874671 1 5.71
CHEMBL4472834 1 5.59
CHEMBL4877300 1 5.49
CHEMBL4877023 1 5.42
CHEMBL4847771 1 5.38
CHEMBL4877805 1 5.36
CHEMBL4796066 1 5.26
CHEMBL4858210 1 5.18
CHEMBL4859992 1 5.08
CHEMBL98 VORINOSTAT 4.0 1 4.80
CHEMBL4473451 1 4.56
CHEMBL4545246 1 4.53
CHEMBL4438566 1 4.44
CHEMBL4846564 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4530950 IC50 = 350.0 nM 6.46
CHEMBL4862510 IC50 = 590.0 nM 6.23
CHEMBL4872697 IC50 = 720.0 nM 6.14
CHEMBL4852441 IC50 = 730.0 nM 6.14
CHEMBL4874716 IC50 = 850.0 nM 6.07
CHEMBL4861336 IC50 = 900.0 nM 6.05
CHEMBL4874671 IC50 = 1960.0 nM 5.71
CHEMBL4472834 IC50 = 2580.0 nM 5.59
CHEMBL4877300 IC50 = 3260.0 nM 5.49
CHEMBL4877023 IC50 = 3820.0 nM 5.42
CHEMBL4847771 IC50 = 4200.0 nM 5.38
CHEMBL4877805 IC50 = 4340.0 nM 5.36
CHEMBL4796066 IC50 = 5510.0 nM 5.26
CHEMBL4858210 IC50 = 6560.0 nM 5.18
CHEMBL4859992 IC50 = 8270.0 nM 5.08
CHEMBL98 VORINOSTAT IC50 = 15830.0 nM 4.80
CHEMBL4473451 IC50 = 27570.0 nM 4.56
CHEMBL4545246 IC50 = 29700.0 nM 4.53
CHEMBL4438566 IC50 = 36470.0 nM 4.44
CHEMBL4846564 IC50 > 40000.0 nM -