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Assay Detail

CHEMBL4823511

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of pig pancreatic lipase type 2 using p-nitrophenyl butyrate as substrate preincubated with enzyme for 10 mins followed by susbtrate addition and measured after 30 mins by spectrophotometric analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Natural Isoflavones and Semisynthetic Derivatives as Pancreatic Lipase Inhibitors.
Cardullo N, Muccilli V, Pulvirenti L, Tringali C.
J Nat Prod (2021) 84 : 654 -665
PubMed 33646787 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.33 6.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL175247 ORLISTAT 4.0 1 6.22
CHEMBL44 GENISTEIN 2.0 1 4.22
CHEMBL4780482 1 4.20
CHEMBL13486 1 4.16
CHEMBL8145 DAIDZEIN 2.0 1 4.08
CHEMBL4878391 1 4.06
CHEMBL4870006 1 4.04
CHEMBL1290231 1 4.03
CHEMBL4859886 1 4.00
CHEMBL242341 FORMONONETIN 2.0 1 -
CHEMBL273891 1 -
CHEMBL4874262 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL175247 ORLISTAT IC50 = 600.0 nM 6.22
CHEMBL44 GENISTEIN IC50 = 60000.0 nM 4.22
CHEMBL4780482 IC50 = 63000.0 nM 4.20
CHEMBL13486 IC50 = 70000.0 nM 4.16
CHEMBL8145 DAIDZEIN IC50 = 83000.0 nM 4.08
CHEMBL4878391 IC50 = 87000.0 nM 4.06
CHEMBL4870006 IC50 = 92000.0 nM 4.04
CHEMBL1290231 IC50 = 93000.0 nM 4.03
CHEMBL4859886 IC50 = 100000.0 nM 4.00
CHEMBL242341 FORMONONETIN IC50 = 120000.0 nM -
CHEMBL273891 IC50 = 360000.0 nM -
CHEMBL4874262 IC50 = 122000.0 nM -