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Assay Detail

CHEMBL4823993

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against HCV 1b infected in human Huh-7 cells assessed as reduction in viral replication measured after 3 days by dual-Glo luciferase assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Hepacivirus hominis
Cell Type Huh-7
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatitis C virus (CHEMBL379)
Type ORGANISM
Organism Hepacivirus hominis

Publication

Prodrugs of a 1'-CN-4-Aza-7,9-dideazaadenosine <i>C</i>-Nucleoside Leading to the Discovery of Remdesivir (GS-5734) as a Potent Inhibitor of Respiratory Syncytial Virus with Efficacy in the African Green Monkey Model of RSV.
Mackman RL, Hui HC, Perron M, Murakami E, Palmiotti C, Lee G, Stray K, Zhang L, Goyal B, Chun K, Byun D, Siegel D, Simonovich S, Du Pont V, Pitts J, Babusis D, Vijjapurapu A, Lu X, Kim C, Zhao X, Chan J, Ma B, Lye D, Vandersteen A, Wortman S, Barrett KT, Toteva M, Jordan R, Subramanian R, Bilello JP, Cihlar T.
J Med Chem (2021) 64 : 5001 -5017
PubMed 33835812 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.09 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4079082 1 8.46
CHEMBL2016757 GS-441524 1.0 1 5.39
CHEMBL2016758 1 4.41
CHEMBL2016760 1 -
CHEMBL3120779 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4079082 EC50 = 3.5 nM 8.46
CHEMBL2016757 GS-441524 EC50 = 4100.0 nM 5.39
CHEMBL2016758 EC50 = 39000.0 nM 4.41
CHEMBL2016760 EC50 > 89000.0 nM -
CHEMBL3120779 EC50 > 89000.0 nM -