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Assay Detail

CHEMBL4824378

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full length GSK3beta expressed in baculovirus in Sf9 insect cells using YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE as substrate by ADP-Glo kinase assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glycogen synthase kinase-3 beta (CHEMBL262)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of harmine derivatives as potent GSK-3β/DYRK1A dual inhibitors for the treatment of Alzheimer's disease.
Liu W, Liu X, Tian L, Gao Y, Liu W, Chen H, Jiang X, Xu Z, Ding H, Zhao Q.
Eur J Med Chem (2021) 222 : 113554 -113554
PubMed 34098466 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.03 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL388978 STAUROSPORINE 1 7.52
CHEMBL322970 1 7.27
CHEMBL4849247 1 7.15
CHEMBL4846517 1 6.84
CHEMBL4872945 1 6.70
CHEMBL4873680 1 6.40
CHEMBL4871399 1 5.32
CHEMBL4854952 1 5.29
CHEMBL4845857 1 5.18
CHEMBL4878234 1 5.12
CHEMBL4869592 1 5.09
CHEMBL269538 HARMINE 1.0 1 4.49
CHEMBL4859020 1 -
CHEMBL4875836 1 -
CHEMBL4854983 1 -
CHEMBL4878139 1 -
CHEMBL4848873 1 -
CHEMBL4870826 1 -
CHEMBL4863925 1 -
CHEMBL4868963 1 -
CHEMBL4875404 1 -
CHEMBL4866620 1 -
CHEMBL4861340 1 -
CHEMBL4861480 1 -
CHEMBL4860218 1 -
CHEMBL4856645 1 -
CHEMBL4873051 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL388978 STAUROSPORINE IC50 = 30.0 nM 7.52
CHEMBL322970 IC50 = 54.0 nM 7.27
CHEMBL4849247 IC50 = 71.0 nM 7.15
CHEMBL4846517 IC50 = 144.0 nM 6.84
CHEMBL4872945 IC50 = 200.0 nM 6.70
CHEMBL4873680 IC50 = 400.0 nM 6.40
CHEMBL4871399 IC50 = 4800.0 nM 5.32
CHEMBL4854952 IC50 = 5150.0 nM 5.29
CHEMBL4845857 IC50 = 6590.0 nM 5.18
CHEMBL4878234 IC50 = 7620.0 nM 5.12
CHEMBL4869592 IC50 = 8100.0 nM 5.09
CHEMBL269538 HARMINE IC50 = 32100.0 nM 4.49
CHEMBL4859020 IC50 > 10000.0 nM -
CHEMBL4875836 IC50 > 10000.0 nM -
CHEMBL4854983 IC50 > 10000.0 nM -
CHEMBL4878139 IC50 > 10000.0 nM -
CHEMBL4848873 IC50 > 10000.0 nM -
CHEMBL4870826 IC50 > 10000.0 nM -
CHEMBL4863925 IC50 > 10000.0 nM -
CHEMBL4868963 IC50 > 10000.0 nM -
CHEMBL4875404 IC50 > 10000.0 nM -
CHEMBL4866620 IC50 > 10000.0 nM -
CHEMBL4861340 IC50 > 10000.0 nM -
CHEMBL4861480 IC50 > 10000.0 nM -
CHEMBL4860218 IC50 > 10000.0 nM -
CHEMBL4856645 IC50 > 10000.0 nM -
CHEMBL4873051 IC50 > 10000.0 nM -