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Assay Detail

CHEMBL4825988

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of P-gp (unknown origin) expressed in MES-SA/DX5 cells assessed as cell growth inhibition after 3 days in presence of 200 nM paclitaxel by MTT assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MES-SA/Dx5
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Encequidar, First-in-Class Intestine Specific P-glycoprotein Inhibitor.
Smolinski MP, Urgaonkar S, Pitzonka L, Cutler M, Lee G, Suh KH, Lau JYN.
J Med Chem (2021) 64 : 3677 -3693
PubMed 33729781 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 7.60 7.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4594298 ENCEQUIDAR 3.0 1 7.89
CHEMBL348475 TARIQUIDAR 3.0 1 7.80
CHEMBL4873470 1 7.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4594298 ENCEQUIDAR EC50 = 13.0 nM 7.89
CHEMBL348475 TARIQUIDAR EC50 = 16.0 nM 7.80
CHEMBL4873470 EC50 = 75.0 nM 7.12